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A genome-wide study of recombination rate variation in Bartonella henselae
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
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2012 (English)In: BMC Evolutionary Biology, ISSN 1471-2148, E-ISSN 1471-2148, Vol. 12, 65- p.Article in journal (Refereed) Published
Abstract [en]

Background: Rates of recombination vary by three orders of magnitude in bacteria but the reasons for this variation is unclear. We performed a genome-wide study of recombination rate variation among genes in the intracellular bacterium Bartonella henselae, which has among the lowest estimated ratio of recombination relative to mutation in prokaryotes. Results: The 1.9 Mb genomes of B. henselae strains IC11, UGA10 and Houston-1 genomes showed only minor gene content variation. Nucleotide sequence divergence levels were less than 1% and the relative rate of recombination to mutation was estimated to 1.1 for the genome overall. Four to eight segments per genome presented significantly enhanced divergences, the most pronounced of which were the virB and trw gene clusters for type IV secretion systems that play essential roles in the infection process. Consistently, multiple recombination events were identified inside these gene clusters. High recombination frequencies were also observed for a gene putatively involved in iron metabolism. A phylogenetic study of this gene in 80 strains of Bartonella quintana, B. henselae and B. grahamii indicated different population structures for each species and revealed horizontal gene transfers across Bartonella species with different host preferences. Conclusions: Our analysis has shown little novel gene acquisition in B. henselae, indicative of a closed pan-genome, but higher recombination frequencies within the population than previously estimated. We propose that the dramatically increased fixation rate for recombination events at gene clusters for type IV secretion systems is driven by selection for sequence variability.

Place, publisher, year, edition, pages
2012. Vol. 12, 65- p.
Keyword [en]
Type IV Secretion Systems, Recombination, Bartonella
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-185218DOI: 10.1186/1471-2148-12-65ISI: 000310328700001OAI: oai:DiVA.org:uu-185218DiVA: diva2:571097
Available from: 2012-11-21 Created: 2012-11-21 Last updated: 2017-12-07Bibliographically approved

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Publisher's full texthttp://www.biomedcentral.com/1471-2148/12/65

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Guy, LionelNäslund, KristinaBerglund, Eva C.Andersson, Siv G. E.
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