TMV-particle borne enhancer of a tobacco RNA-replicase
2012 (English)In: World Journal of Science and Technology, ISSN 2231-2587, Vol. 2, no 7, 4-7 p.Article in journal (Refereed) Published
According to separation studies it has been evident that a tobacco RNA-replicase after TMV-infection consists of two parts. The larger part is host-directed and will be combined with a virus borne small protein which considerably enhances the RNA-replicase activity and is therefore named replicase enhancer, Ree. This compound was found at HPLC-separations of TMV-coat proteins, and was detected using polymerase assay with a radioactive nucleotide involved. Molecular weight has been determined by mass spectrometry: with FT ICR MS to get the size – 6 023.3 - and with MALDI TOF MS to obtain a sequence of the 54 amino acids involved. Presumably Ree is fixed to the TMV-RNA at infection. The enzyme seems to produce minus-strands of the virus RNA.
Place, publisher, year, edition, pages
2012. Vol. 2, no 7, 4-7 p.
Enhancer, mass spectrometry, replicase, TMV, tobacco
IdentifiersURN: urn:nbn:se:uu:diva-184707OAI: oai:DiVA.org:uu-184707DiVA: diva2:567466