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TMV-particle borne enhancer of a tobacco RNA-replicase
MASE Laboratory, Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
2012 (English)In: World Journal of Science and Technology, ISSN 2231-2587, Vol. 2, no 7, 4-7 p.Article in journal (Refereed) Published
Abstract [en]

According to separation studies it has been evident that a tobacco RNA-replicase after TMV-infection consists of two parts. The larger part is host-directed and will be combined with a virus borne small protein which considerably enhances the RNA-replicase activity and is therefore named replicase enhancer, Ree. This compound was found at HPLC-separations of TMV-coat proteins, and was detected using polymerase assay with a radioactive nucleotide involved. Molecular weight has been determined by mass spectrometry: with FT ICR MS to get the size – 6 023.3 - and with MALDI TOF MS to obtain a sequence of the 54 amino acids involved. Presumably Ree is fixed to the TMV-RNA at infection. The enzyme seems to produce minus-strands of the virus RNA.

Place, publisher, year, edition, pages
2012. Vol. 2, no 7, 4-7 p.
Keyword [en]
Enhancer, mass spectrometry, replicase, TMV, tobacco
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-184707OAI: oai:DiVA.org:uu-184707DiVA: diva2:567466
Available from: 2012-11-15 Created: 2012-11-13 Last updated: 2012-11-15Bibliographically approved

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Hanrieder, JörgBergquist, Jonas

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