Colocalization Analysis in Fluorescence Microscopy
2012 (English)In: Cell Imaging Techniques: Methods and Protocols / [ed] Taatjes, Douglas J. & Roth, Jürgen, New York: Humana Press, 2012, 97-109 p.Chapter in book (Refereed)
The measurement of colocalization requires images of two fluorophores that are aligned, with no cross talk, and that the intensities remain within the response range of the microscope. Quantitation depends upon differentiating between the presence and absence of fluorescence, and measurements should be made within biologically relevant regions of interest. Co-occurrence can be measured simply by area or with the M1 and M2 coefficients, and should be compared to random distributions. Correlation analysis should use the Pearson and Spearman coefficients, which need to be measured by replicate based noise corrected correlation to eliminate errors arising from differences in image quality. Ideally, both co-occurrence and correlation should be reported.
Place, publisher, year, edition, pages
New York: Humana Press, 2012. 97-109 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 319
colocalization, co-occurrence, correlation, pearson correlation coefficient, RBNCC
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-183653DOI: 10.1007/978-1-62703-056-4_5ISBN: 978-1-62703-055-7OAI: oai:DiVA.org:uu-183653DiVA: diva2:563664