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Regulation of Aurora A activity during checkpoint recovery
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. (Arne Lindqvist (Department of Cell and Molecular Biology, Karolinska Institutet))
2012 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Cell division requires accurate DNA replication and cells develop checkpoint mechanisms toensure the correct passage of the genetic material. Cells arrest by a checkpoint when DNAdamage is found. After the checkpoint is silenced, the cell cycle can be resumed. Polo-likekinase 1 (Plk1) and Aurora A kinase (AurA) are both important regulators for checkpointrecovery. The question how AurA is activated was studied by many researchers, but the exactmechanism stays unclear.We developed a new setup to study AurA activation during checkpoint recovery. Quantitativeimmunofluorescence of fixed cells as well as a FRET probe that monitors Plk1 activity intime-lapse filming were applied in this study as indirect readouts of Aurora A activation. Theresult suggests that a Plk1-AurA feedback loop exists during checkpoint recovery. It can alsobe concluded that the inhibition of Cdk1 reduces Plk1 and AurA activity during checkpointrecovery. We also investigated the effect of calcium interfering drugs on AurA activation butno conclusive result was obtained.

Place, publisher, year, edition, pages
2012. , 33 p.
Keyword [en]
AurA activation, G2 checkpoint, checkpoint recovery, Time-lapse FRET microscopy
National Category
Cell Biology
URN: urn:nbn:se:uu:diva-181746OAI: diva2:557463
Educational program
Master Programme in Biology
2012-09-27, B1:120, Uppsala biomedicinska centrum, Uppsala, 23:19 (English)
Available from: 2012-09-28 Created: 2012-09-27 Last updated: 2012-09-28Bibliographically approved

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