Change search
ReferencesLink to record
Permanent link

Direct link
Effect of 5-Flourouracil On HCT-116 P53+/- Human Colon Cancer Cells
University of Skövde, School of Life Sciences.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

 The purpose of this study was to observe the effects of 5-flourouracil on HCT-116 p53 +/- colon cancer cells. In order to investigate the effects of the drug, concentration-dependent assays and time-dependent  assay  procedures  were  used.  To  evaluate  the  cell  viability  after  being  treated with  the  drug,  XTT  technique  was  used.  After  performing  concentration-dependent  assays  a consequent  decrease  in  cell  viability  was  observed  with  increase  in  concentration  of  the  drug. The time-dependent assay showed a gradual decrease in cell viability with increase in time after exposure to the drug. Western blotting technique was performed on the proteins extracted from the samples. Expression of Bak was examined after the cells were exposed to the drug. Results from western blotting technique revealed that Bak protein was initially over-expressed after the cells were treated with 5-Flourouracil for 48 hours and this over-expression of the Bak resulted in   apoptosis   of   cancer   cells.   Thus,   5-Flourouracil   is   considered   to   be   an   effective chemotherapeutic  drug  for  colon  cancer  cells  partially  though  the  over-expression  of  Bak protein.

Place, publisher, year, edition, pages
2011. , 30 p.
National Category
Natural Sciences
URN: urn:nbn:se:his:diva-6351OAI: diva2:548807
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Master's Programme
Available from: 2013-04-12 Created: 2012-09-01 Last updated: 2013-04-12Bibliographically approved

Open Access in DiVA

fulltext(934 kB)175 downloads
File information
File name FULLTEXT01.pdfFile size 934 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
School of Life Sciences
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 175 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 47 hits
ReferencesLink to record
Permanent link

Direct link