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MEASUREMENT OF C-REACTIVE PROTEIN IN CANINE SERUM ON KONELABAUTOANALYZER 20
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2012 (Swedish)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

An inflammatory reaction is induced after release of proinflammatory mediators such asinterleukin 1 and 6 and tumour necrosis factor α. These mediators stimulate the liver tosuppress the syntheses of albumin and endure the syntheses of acute phase protein forinstance C-reactive protein. The aim of this paper was to perform a method validation on animmune turbidimetric assay to quantify C-reactive protein in canine serum at the laboratory atSkara Animals Hospital, Skara, Sweden. The validation involved evaluation of the assaylinearity, precision, stability and recovery.The method was proved to be linear for both TruLab control and Medinor control. TheTruLab control is based on human C-reactive protein while the Medinor control is based oncanine C-reactive protein. The precision of the method had a CV of 2.26 % (n=40). Themethod is considered stable and has good precision, under the circumstance that the TruLabcontrol is used. It was concluded that a Canine CRP-control is required and should beincluded in routine analysis.

Place, publisher, year, edition, pages
2012. , 11 p.
Keyword [en]
Immunoturbidimetric, Serum-concentration, Canine, Inflammatory, Acutephaseprotein.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-179622OAI: oai:DiVA.org:uu-179622DiVA: diva2:545564
External cooperation
Skara Djursjukhus
Educational program
Biomedicinska analytikerprogrammet
Uppsok
Medicine
Supervisors
Examiners
Available from: 2012-08-21 Created: 2012-08-20 Last updated: 2012-08-21Bibliographically approved

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