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Genotyping should be considered the primary choice for pre-treatment evaluation of thiopurine methyltransferase function
Department of Gastroenterology, Skåne University Hospital, Lund, Sweden.
Linköping University, Department of Medical and Health Sciences, Clinical Pharmacology. Linköping University, Faculty of Health Sciences.ORCID iD: 0000-0002-2809-7591
2012 (English)In: Journal of Crohn's and Colitis, ISSN 1873-9946, Vol. 6, no 6, 655-659 p.Article in journal (Refereed) Published
Abstract [en]

Background and aims

A pre-treatment determination of the thiopurine S-methyltransferase (TPMT) genotype or phenotype can identify patients at risk of developing severe adverse reactions from thiopurine treatment. The risk of misclassifying a patient might be dependent on the method used. The aim of this study was to investigate the concordance between TPMT genotyping and phenotyping.

Methods

The data consist of 7195 unselected and consecutive TPMT genotype and phenotype determinations sent to the division of Clinical Pharmacology, Linköping, Sweden. TPMT activity was measured in red blood cells (RBC) and the genotype determined by pyrosequencing for the three most common TPMT variants (TPMT *2, *3A, *3C).

Results

TPMT genotyping identified 89% as TPMT wild type (*1/*1), 10% as TPMT heterozygous and 0.5% as TMPT defective. The overall concordance between genotyping and phenotyping was 95%, while it was 96% among IBD patients (n = 4024). Genotyping would have misclassified 8% of the TPMT defectives as heterozygous as compared to 11% if only TPMT activity had been measured. 11% of the heterozygous patients had a normal TPMT activity (> 8.9 U/ml RBC) and 3% of the TPMT wild-type patients had an intermediate TPMT activity (2.5–8.9 U/ml RBC).

Conclusions

There is a risk for TPMT misclassification when only genotyping or phenotyping is used, but it is not reasonable to check both in all patients. Since TPMT genotyping is the more reliable test, especially in TPMT heterozygotes, we suggest that genotyping should be considered the primary choice for the pre-treatment evaluation of TPMT function before initiation of thiopurine therapy.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 6, no 6, 655-659 p.
Keyword [en]
Thiopurine methyltransferase, Thiopurines, Inflammatory bowel diseases, Genotype, Phenotype
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-79120DOI: 10.1016/j.crohns.2011.11.014ISI: 000305874000003OAI: oai:DiVA.org:liu-79120DiVA: diva2:538370
Available from: 2012-06-29 Created: 2012-06-29 Last updated: 2013-09-03

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