Bacterial Sortase A as a drug target
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Sortase A is a housekeeping enzyme of Gram-positive bacteria that catalyses the anchoring of surface proteins to the bacterial peptidoglycan. The enzyme works to establish an interaction between bacteria and host cells and is essential for pathogenesis. This makes Sortase A a potential suitable target for inhibition, in order to treat bacterial infections.
In this degree project Sortase A from Staphylococcus aureus was explored and potential inhibitors were investigated by performing enzyme activity and bacterial binding assays. A robust FRET assay was developed and optimized for a recombinant version of the enzyme and serves as a good starting point for studying inhibition.
Place, publisher, year, edition, pages
2012. , 49 p.
Antibiotic resistance, Sortase A, Staphylococcus aureus, Gram-positive bacteria, inhibition, FRET assay, fibronectin-binding
Biocatalysis and Enzyme Technology
IdentifiersURN: urn:nbn:se:uu:diva-176862OAI: oai:DiVA.org:uu-176862DiVA: diva2:537309
Molecular Biotechnology Engineering Programme