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Bacterial Sortase A as a drug target
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2012 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Sortase A is a housekeeping enzyme of Gram-positive bacteria that catalyses the anchoring of surface proteins to the bacterial peptidoglycan. The enzyme works to establish an interaction between bacteria and host cells and is essential for pathogenesis. This makes Sortase A a potential suitable target for inhibition, in order to treat bacterial infections.

In this degree project Sortase A from Staphylococcus aureus was explored and potential inhibitors were investigated by performing enzyme activity and bacterial binding assays. A robust FRET assay was developed and optimized for a recombinant version of the enzyme and serves as a good starting point for studying inhibition.

Place, publisher, year, edition, pages
2012. , 49 p.
Keyword [en]
Antibiotic resistance, Sortase A, Staphylococcus aureus, Gram-positive bacteria, inhibition, FRET assay, fibronectin-binding
National Category
Biocatalysis and Enzyme Technology
URN: urn:nbn:se:uu:diva-176862OAI: diva2:537309
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2012-06-26 Created: 2012-06-26 Last updated: 2012-06-26Bibliographically approved

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Biocatalysis and Enzyme Technology

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