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Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Department of Neurology, Albert Szent-Györgyi Clinical Center, University of Szeged, Hungary.
Department of Neurology, Albert Szent-Györgyi Clinical Center, University of Szeged, Hungary AND Department of Clinical Neurophysiology, Danish Epilepsy Centre, Denmark.
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2012 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 13, no 6, 7676-7693 p.Article in journal (Refereed) Published
Abstract [en]

Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient’s death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.

Place, publisher, year, edition, pages
2012. Vol. 13, no 6, 7676-7693 p.
Keyword [en]
multiple sclerosis, fulminant, shotgun-proteomics, protein quantification, isobaric tag labeling, matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF/TOF MS)
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-176842DOI: 10.3390/ijms13067676ISI: 000306188700074OAI: oai:DiVA.org:uu-176842DiVA: diva2:537195
Available from: 2012-06-26 Created: 2012-06-26 Last updated: 2017-12-07Bibliographically approved

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