Enzyme Linked Immuno-Spot; a Useful Tool in the Search for Elusive Immune Markers in Common Pediatric Immunological Diseases
2012 (English)In: Cells, ISSN 2073-4409, Vol. 1, no 2, 141-152 p.Article, review/survey (Refereed) Published
In order to provide better therapy we strive to increase our knowledge of how the immune system behaves and communicates in common pediatric immunological diseases, such as type 1 diabetes, allergic and celiac diseases. However, when dealing with pediatric diseases, where study subjects are almost exclusively children, blood volumes available for immunological studies are limited and as such must be carefully handled and used to their full extent. Single immune markers can easily be detected by a traditional Enzyme Linked Immunosorbent Assay (ELISA), whereas multiple markers can be detected by a fluorochrome (Luminex) or electrochemiluminescence (MSD) technique. These techniques however are sometimes not sensitive enough to detect low levels of secreted immune markers in limited sample sizes. To detect immune markers at the single-cell level, an Enzyme Linked Immuno-spot (ELISPOT) can be used to pin-point elusive immune markers in common pediatric immunological diseases.
Place, publisher, year, edition, pages
2012. Vol. 1, no 2, 141-152 p.
Enzyme Linked Immuno-spot (ELISPOT), pediatric immunological diseases, immune markers, cytokines
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:hj:diva-18321DOI: 10.3390/cells1020141OAI: oai:DiVA.org:hj-18321DiVA: diva2:531524