Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Wai)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Wai)
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Show others and affiliations
2012 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 80, no 2, 575-584 p.Article in journal (Refereed) Published
Abstract [en]

The most recently discovered secretion pathway in gram-negative bacteria, the type VI secretion system (T6SS), is present in many species and is considered important for the survival of non-O1 non-O139 Vibrio cholerae in aquatic environments. Until now, it was not known whether there is a functionally active T6SS in wild-type V. cholerae O1 strains, the cause of cholera disease in humans. Here, we demonstrate the presence of a functionally active T6SS in wild-type V. cholerae O1 strains, as evidenced by the secretion of the T6SS substrate Hcp, which required several gene products encoded within the putative vas gene cluster. Our analyses showed that the T6SS of wild-type V. cholerae O1 strain A1552 was functionally activated when the bacteria were grown under high-osmolarity conditions. The T6SS was also active when the bacteria were grown under low temperature (23°C), suggesting that the system may be important for the survival of the bacterium in the environment. A test of the interbacterial virulence of V. cholerae strain A1552 against an Escherichia coli K-12 strain showed that it was strongly enhanced under high osmolarity and that it depended on the hcp genes. Interestingly, we found that the newly recognized osmoregulatory protein OscR plays a role in the regulation of T6SS gene expression and secretion of Hcp from V. cholerae O1 strains.

Place, publisher, year, edition, pages
American Society for Microbiology , 2012. Vol. 80, no 2, 575-584 p.
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-55878DOI: 10.1128/IAI.05510-11PubMedID: 22083711OAI: oai:DiVA.org:umu-55878DiVA: diva2:531516
Available from: 2012-06-08 Created: 2012-06-07 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

fulltext(1479 kB)612 downloads
File information
File name FULLTEXT02.pdfFile size 1479 kBChecksum SHA-512
4dbff58aadd6ef8bf3957249aec6bf6f35288076737464e3c90723f6cc8f110094910bfb3fcbfbee3a9330333dbdee1b61e3f584d79eb69f6b3a07024e7e0ff3
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ishikawa, TakahikoSabharwal, DharmeshBröms, JeanetteMilton, Debra LSjöstedt, AndersUhlin, Bernt EricWai, Sun Nyunt
By organisation
Department of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)Clinical BacteriologyMolecular Infection Medicine Sweden (MIMS)
In the same journal
Infection and Immunity
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 612 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 285 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf