Change search
ReferencesLink to record
Permanent link

Direct link
Studies on the Restriction of Murine Leukemia Viruses by Mouse APOBEC3
Show others and affiliations
2012 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 7, no 5, e38190- p.Article in journal (Refereed) Published
Abstract [en]

APOBEC3 proteins function to restrict the replication of retroviruses. One mechanism of this restriction is deamination of cytidines to uridines in (-) strand DNA, resulting in hypermutation of guanosines to adenosines in viral (+) strands. However, Moloney murine leukemia virus (MoMLV) is partially resistant to restriction by mouse APOBEC3 (mA3) and virtually completely resistant to mA3-induced hypermutation. In contrast, the sequences of MLV genomes that are in mouse DNA suggest that they were susceptible to mA3-induced deamination when they infected the mouse germline. We tested the possibility that sensitivity to mA3 restriction and to deamination resides in the viral gag gene. We generated a chimeric MLV in which the gag gene was from an endogenous MLV in the mouse germline, while the remainder of the viral genome was from MoMLV. This chimera was fully infectious but its response to mA3 was indistinguishable from that of MoMLV. Thus, the Gag protein does not seem to control the sensitivity of MLVs to mA3. We also found that MLVs inactivated by mA3 do not synthesize viral DNA upon infection; thus mA3 restriction of MLV occurs before or at reverse transcription. In contrast, HIV-1 restricted by mA3 and MLVs restricted by human APOBEC3G do synthesize DNA; these DNAs exhibit APOBEC3-induced hypermutation.

Place, publisher, year, edition, pages
2012. Vol. 7, no 5, e38190- p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-175424DOI: 10.1371/journal.pone.0038190ISI: 000305349600070PubMedID: 22666481ISBN: 1932-6203 (Electronic) 1932-6203 (Linking)OAI: diva2:531374
Available from: 2012-06-07 Created: 2012-06-07 Last updated: 2012-07-17Bibliographically approved

Open Access in DiVA

fulltext(1280 kB)215 downloads
File information
File name FULLTEXT01.pdfFile size 1280 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Jern, Patric
By organisation
Department of Medical Biochemistry and MicrobiologyScience for Life Laboratory, SciLifeLab
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 215 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 334 hits
ReferencesLink to record
Permanent link

Direct link