Prokaryotic community structure and respiration during long-term incubations
2012 (English)In: MicrobiologyOpen, ISSN 2045-8827, Vol. 1, no 2, 214-224 p.Article in journal (Refereed) Published
Despite the importance of incubation assays for studies inmicrobial ecology that frequentlyrequire long confinement times, few reports are available in which changesin the assemblage structure of aquatic prokaryotes were monitored during longtermincubations.We measured rates of dissolved organic carbon degradation andmicrobial respiration by consumption of dissolved oxygen (DO) in four experimentswith Lake Kinneret near-surface water and, concomitantly, we analyzed thevariability in prokaryotic community structure during long-term dark bottle incubations.During the first 24 h, therewere only minor changes in bacterial communitycomposition. Thereafter there were marked changes in the prokaryotic communitystructure during the incubations. In contrast, oxygen consumption rates (a proxyfor both respiration and dissolved organic carbon degradation rates) remained stablefor up to 10–23 days. This study is one of the first to examine closely the phylogeneticchanges that occur in the microbial community of untreated freshwaterduring long-term (days) incubations in dark, sealed containers. Novel informationon the diversity of the main bacterial phylotypes that may be involved in dissolvedorganic matter degradation in lake Kinneret is also provided. Our results suggestthat, under certain ecological settings, constant community metabolic rates can bemaintained as a result of shifts in community composition.
Place, publisher, year, edition, pages
2012. Vol. 1, no 2, 214-224 p.
Research subject Natural Science, Aquatic Ecology
IdentifiersURN: urn:nbn:se:lnu:diva-18935DOI: 10.1002/mbo3.25OAI: oai:DiVA.org:lnu-18935DiVA: diva2:528725