Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Protein import into peroxisomes and oxidative stress: a study to elucidate the potential functional role of the conserved cysteine in Pex5p
University of Skövde, School of Life Sciences.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The oxidation status of a cell plays a crucial role in aging. As cells get aged, their redox state gets increased. Pex5p is a peroxisomal recycling receptor which binds to newly synthesized cargo proteins in the cytosol and imports them across the peroxisomal membrane. During this transport event, Pex5p gets monoubiquitinated at a conserved cysteine (C11) residue. This C11 is very essential for the recycling of Pex5p from the peroxisomal membrane to back into the cytosol. If the cysteine is replaced by serine, Pex5p does not get recycled back to the cytosol and accumulates on the peroxisomal membrane. In the present study, we have investigated whether the C11 in Pex5p could act as a redox switch. We measured the redox state of the cytosol and the peroxisomal matrix as well as the subcellular localization of catalase in aging cells. We found that an increase in the redox state of peroxisomes (in WT) leads to an increase in the redox state of the cytosol, which ultimately results in the impairment of PTS1 import. Interestingly, in the C11K condition, we did not see an impairment of PTS1 import. These observations support our hypothesis that C11 may act as a redox switch. We also performed some challenging experiments with H2O2. The results of these experiments show that a) import of catalase into peroxisomes sensitizes the cytosol and b) catalase overexpression does have a protective effect against oxidative stress caused by H2O2. In summary the results of our experiments support our hypothesis. However, further evaluation is needed to reveal the precise role of C11 in Pex5p function during cellular aging. 

Place, publisher, year, edition, pages
2011. , 36 p.
Keyword [en]
Peroxisomes, Pex5p, PTS1 import, ROS, Catalase, H2O2
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:his:diva-5888OAI: oai:DiVA.org:his-5888DiVA: diva2:527814
External cooperation
KU Leuven, Belgium
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Master's Programme
Presentation
2012-03-27, 13:00 (English)
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2012-05-25 Created: 2012-05-22 Last updated: 2012-05-25Bibliographically approved

Open Access in DiVA

Master thesis(1496 kB)419 downloads
File information
File name FULLTEXT01.pdfFile size 1496 kBChecksum SHA-512
3d1f3b92aea46524f6a6620d0ca23a9510cff920bcc01fbceb9404af2a0a0fd93f12b3b345f294cb27de6448d7609f6b4bd6222261ca6be91442dfdc2c28500d
Type fulltextMimetype application/pdf

By organisation
School of Life Sciences
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 419 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 121 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf