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An Electrochemical Immunoassay for HER2 Detection
University of Florence.
University of Florence.
University of Florence.
University of Florence.
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2012 (English)In: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 24, no 4, 735-742 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.

Place, publisher, year, edition, pages
Wiley-VCH Verlag Berlin , 2012. Vol. 24, no 4, 735-742 p.
Keyword [en]
HER2, Cancer marker, Magnetic beads, Immunoassay
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-76937DOI: 10.1002/elan.201100501ISI: 000302160000005OAI: diva2:524588
Available from: 2012-05-03 Created: 2012-04-27 Last updated: 2013-10-04

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