Combinatorial Activation and Repression by Seven Transcription Factors Specify Drosophila Odorant Receptor Expression
2012 (English)In: PLoS biology, ISSN 1544-9173, E-ISSN 1545-7885, Vol. 10, no 3, e1001280- p.Article in journal (Refereed) Published
The mechanism that specifies olfactory sensory neurons to express only one odorant receptor (OR) from a large repertoire is critical for odor discrimination but poorly understood. Here, we describe the first comprehensive analysis of OR expression regulation in Drosophila. A systematic, RNAi-mediated knock down of most of the predicted transcription factors identified an essential function of acj6, E93, Fer1, onecut, sim, xbp1, and zf30c in the regulation of more than 30 ORs. These regulatory factors are differentially expressed in antennal sensory neuron classes and specifically required for the adult expression of ORs. A systematic analysis reveals not only that combinations of these seven factors are necessary for receptor gene expression but also a prominent role for transcriptional repression in preventing ectopic receptor expression. Such regulation is supported by bioinformatics and OR promoter analyses, which uncovered a common promoter structure with distal repressive and proximal activating regions. Thus, our data provide insight into how combinatorial activation and repression can allow a small number of transcription factors to specify a large repertoire of neuron classes in the olfactory system.
Place, publisher, year, edition, pages
Public Library of Science , 2012. Vol. 10, no 3, e1001280- p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-76960DOI: 10.1371/journal.pbio.1001280ISI: 000302239700004OAI: oai:DiVA.org:liu-76960DiVA: diva2:524000
Funding Agencies|Marie Curie Actions (European Commission)||Swedish Research Council||Swedish Strategic Research Foundation||Boehringer Ingelheim GmbH||DFG||Schram-Foundation||2012-04-272012-04-272015-09-02