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Embryonic Gene Alterations in rats Caused by Exposure to Diabetes and/or Obesity
University of Skövde, School of Life Sciences. (Ulf J Eriksson´s group)
2012 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

There is ample evidence that both diabetes as well as obesity leads to various metabolic disturbances that leads to oxidative stress. Oxidative stress has been shown  to  be  associated  with  congenital  malformations  of  which  neural  tube defects  and  cardiac  malformations  are  more  common.  The  cellular  and molecular  mechanisms  through  which  oxidative  stress  induces  these  defects during the developmental stage are not well known. Previous work in this field suggests   that   oxidative   stress   results   in   lipid   peroxidation   and   altered expression  of  genes  that  have  key  roles  in  the  developmental  processes.  The present study aimed to investigate gene alterations in embryos from pregnant diabetic  or  obese  rats.  Embryos  and  adipose  tissue  obtained  from  the  locally bred  diabetic  and  obese  Sprague-Dawley  inbred  rat  strain  were  subjected  to Total  RNA  extraction  and  were  quantified  using  Real  time  PCR  for  relative gene expressions analysis. The present study showed that maternal diabetes as well  as  obesity  diminishes  the  antioxidative  defense  mechanisms  by  down regulating the gene expressions of the key reactive oxygen species scavenging enzymes   copper   zinc   superoxide   dismutase   and   manganese   superoxide dismutase  in  day  10  rat  embryos.  There  was  also  altered  embryonic  gene expression  for  several  developmental  genes  due  to  maternal  diabetes  at gestational day 11 and 13 in rat embryos.

Place, publisher, year, edition, pages
2012. , 30 p.
National Category
Cell and Molecular Biology
URN: urn:nbn:se:his:diva-5712OAI: diva2:515909
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Master's Programme
2012-03-27, E 103, E building University of Skövde, Skövde, 14:45 (English)
Available from: 2012-04-20 Created: 2012-04-16 Last updated: 2012-04-20Bibliographically approved

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Ehtesham, Ehtesham
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