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Effect of oxaliplatin on HCT116 P53+/- colon cancer cells
University of Skövde, School of Life Sciences.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Oxaliplatin as an effective chemotherapeutic agent in FOLFOX regimens is using to treat colorectal cancer. In this study we investigate cytotoxicity of Oxaliplatin as single chemotherapeutic agent toHCT116P53+/- to identify molecular mechanism of Oxaliplatin action in induction of apoptosis pathway. Oxaliplatin exposure to HCT116P53+/- colorectal cell lines with deficiency of mismatch repair characteristic resulted to decrease the number of viable cells through apoptosis. Effective Oxaliplatin concentrations (IC50) which inhibit 50% of cell viability were determined using XTT method. Standard curve and time-dependent assay performed to confirm IC50 concentration. Western blot analysis demonstrated relocalization of Bax to mitochondria and induction of intrinsic apoptosis pathway resulted Oxaliplatin exposure. Inactivation of Bax in HCT116P53+/- will result significant reduction in number of viable cells following treatment with Oxaliplatin

Place, publisher, year, edition, pages
2011. , 31 p.
Keyword [en]
Oxaliplatin; HCT116 P53+/-; XTT; Bax; Apoptosis
National Category
Medical and Health Sciences
URN: urn:nbn:se:his:diva-5451OAI: diva2:504857
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Master's Programme
2012-01-11, Skovde, 10:30 (English)
Available from: 2012-02-29 Created: 2012-02-22 Last updated: 2012-03-19Bibliographically approved

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