Improvement of a CHO Fed-Batch Process by Fortifying with Plant Peptones
2010 (English)In: Cells and Culture: ESACT Proceedings, 2010, Volume 4, Part 3 / [ed] Noll T, Springer, 2010, 281-284 p.Conference paper (Other academic)
A serum-free fed-batch process was developed for production of a human monoclonal antibody in Chinese hamster ovary (CHO) cells based on Biovitrum’s proprietary low protein serum-free medium without animal derived components (BVT4). The cells were fed with glucose, glutamine and Biovitrum’s proprietary low protein serum-free feed medium without animal derived components enriched with amino acids, vitamins, metal traces, peptones, and biosynthesis precursors. To improve the performance of the fed-batch process, we developed the use of plant peptones by studying the dose and timing of the peptone feeding. Different doses of peptone cocktail and amino acid cocktail, as well as different combinations of pep- tone and amino acid cocktails were first screened in 50 ml filter tubes on an AgCell shaker table. The best combinations were then assessed in spinner and 3 L bioreactor cultures. To reinforce our findings, the antibody-producing CHO cells were adapted to a disclosed serum-free medium DMEM/F12 and the beneficial effects of pep- tones were confirmed in a fed-batch process based on the DMEM/F12 serum-free medium.
Place, publisher, year, edition, pages
Springer, 2010. 281-284 p.
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-86330DOI: 10.1007/978-90-481-3419-9_49OAI: oai:DiVA.org:kth-86330DiVA: diva2:500640
20th European Society for Animal Cell Technology, Dresden, Germany, June 17-20, 2007
QC 201202202012-02-202012-02-132012-02-20Bibliographically approved