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Effect of Peptones and Study of Feeding Strategies in a CHO Based Fed-batch Process for the Production of a Human Monoclonal Antibody
KTH, School of Biotechnology (BIO), Bioprocess Technology. (Cell Technology Group)ORCID iD: 0000-0002-5370-4621
Biovitrum/SOBI, Sweden.
Biovitrum/SOBI, Sweden.
2007 (English)In: Cell Technology for Cell Products: Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005 / [ed] Smith R, Dordrecht, The Netherlands: Springer Netherlands, 2007, 371-374 p.Conference paper, Published paper (Other academic)
Abstract [en]

Eight commercial peptones, derived from plants, were studied for their ability of improving the cell growth and the productivity of a CHO cell line producing a human monoclonal antibody. They were also compared to yeast, lactalbumin and meat derived peptones. Seven plant peptones were selected and further studied in combination by Design of Experiment. The best three peptones were then tested in combinations in fed-batch cultivation. The fed-batch process was based on low concentrations of glucose and glutamine with feeding of amino acids, peptones and feed medium including vitamins, metal traces and biosynthesis precursors. This process was based on Biovitrum protein-free proprietary medium for the base medium and the feeding medium. Different feeding strategies, different peptone combinations and phosphate feeding were studied for their ability to improve the cell density, the cell specific productivity and the cultivation longevity

Place, publisher, year, edition, pages
Dordrecht, The Netherlands: Springer Netherlands, 2007. 371-374 p.
Keyword [en]
peptone, protein hydrolysate, CHO, Chinese Hamster Ovary, fed-batch, feed, DoE, Design of Experiment, antibody, MAb, soy, wheat, rice, pea, yeast, cotton, Primatone, lactalbulmin, phosphate
National Category
Pharmaceutical Biotechnology
Research subject
SRA - Molecular Bioscience
Identifiers
URN: urn:nbn:se:kth:diva-86265DOI: 10.1007/978-1-4020-5476-1_61ISBN: 1-4020-5475-0 (print)OAI: oai:DiVA.org:kth-86265DiVA: diva2:500617
Conference
The 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005
Note
QC 20120214Available from: 2012-02-14 Created: 2012-02-13 Last updated: 2012-02-14Bibliographically approved

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