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Inverse-fluorescence correlation spectroscopy: more information and less labeling
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.ORCID iD: 0000-0002-1850-5440
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
2011 (English)In: Frontiers in Bioscience, ISSN 1093-9946, Vol. s3, 385-392 p.Article in journal (Refereed) Published
Abstract [en]

Inverse-Fluorescence Correlation Spectroscopy(iFCS) is a recently developed modification of standardFCS that allows analysis of particles and biomoleculeswithout labeling. The particles generate no signal; insteadthe signal is generated by a surrounding medium. Particlesdiffusing through the FCS-detection volume displace afraction of the surrounding medium, causing transient dipsin the detected signal. These give information about themobility and concentration of the analyzed particles. Alsolabeled particles can be analyzed, whereby their signal iscross-correlated with that from the surrounding medium(iFCCS). This can give information about the volume of thelabeled particles, or alternatively about the size of thedetection volume. Also the interaction of unlabeledparticles with small, labeled ligands can be analyzed withiFCCS. This allows using cross-correlation as a sensitiveindication of binding, even though only one binding-partneris labeled. This review describes the principles of iFCS andiFCCS and measurements of microspheres dissolved in asurrounding medium containing alexa 488. We also discusspractical considerations, and future possibilities foranalyses of biomolecules.

Place, publisher, year, edition, pages
Frontiers in Bioscience , 2011. Vol. s3, 385-392 p.
Keyword [en]
Inverse-Fluorescence Correlation Spectroscopy, inverse-Fluorescence Cross-Correlation Spectroscopy, Fluoresccence Correlation Spectroscopy, Label-Free, Detection Volume, Particle-Sizing, High- Throughput Screening, Diffusion-Coefficient, Particle Shape, Review
National Category
Natural Sciences
Research subject
SRA - Molecular Bioscience
URN: urn:nbn:se:kth:diva-79214ScopusID: 2-s2.0-79955949362OAI: diva2:495250
QC 20120214Available from: 2012-02-14 Created: 2012-02-08 Last updated: 2014-06-09Bibliographically approved

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