Inverse-fluorescence correlation spectroscopy: more information and less labeling
2011 (English)In: Frontiers in Bioscience, ISSN 1093-9946, Vol. s3, 385-392 p.Article in journal (Refereed) Published
Inverse-Fluorescence Correlation Spectroscopy(iFCS) is a recently developed modification of standardFCS that allows analysis of particles and biomoleculeswithout labeling. The particles generate no signal; insteadthe signal is generated by a surrounding medium. Particlesdiffusing through the FCS-detection volume displace afraction of the surrounding medium, causing transient dipsin the detected signal. These give information about themobility and concentration of the analyzed particles. Alsolabeled particles can be analyzed, whereby their signal iscross-correlated with that from the surrounding medium(iFCCS). This can give information about the volume of thelabeled particles, or alternatively about the size of thedetection volume. Also the interaction of unlabeledparticles with small, labeled ligands can be analyzed withiFCCS. This allows using cross-correlation as a sensitiveindication of binding, even though only one binding-partneris labeled. This review describes the principles of iFCS andiFCCS and measurements of microspheres dissolved in asurrounding medium containing alexa 488. We also discusspractical considerations, and future possibilities foranalyses of biomolecules.
Place, publisher, year, edition, pages
Frontiers in Bioscience , 2011. Vol. s3, 385-392 p.
Inverse-Fluorescence Correlation Spectroscopy, inverse-Fluorescence Cross-Correlation Spectroscopy, Fluoresccence Correlation Spectroscopy, Label-Free, Detection Volume, Particle-Sizing, High- Throughput Screening, Diffusion-Coefficient, Particle Shape, Review
Research subject SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-79214ScopusID: 2-s2.0-79955949362OAI: oai:DiVA.org:kth-79214DiVA: diva2:495250
QC 201202142012-02-142012-02-082014-06-09Bibliographically approved