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Induction and cellular expression of tartrate resistant acid phosphatase during dextran sodium sulphate induced colitis in rats
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences.
2009 (English)In: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 132, no 6, p. 599-612Article in journal (Refereed)
Abstract

The aim of this study was to investigate the

cellular and molecular expression of tartrate resistant acid phosphatase (TRAP) as a marker of activated macrophages in macrophage dependent dextran sulphate sodium (DSS)-induced colitis in rats. In normal colon, TRAP+/CX3CR1+ macrophages were located in the upper part of the lamina propria. In the early stage (day 13) of acute colitis prior to

histopathological changes, induction of the cytokines

TNF, IL-12 and IFNgamma occurred concomitant with

increased mRNA and enzyme activity of TRAP along with

a slight increase of TRAP immunolabelling in macrophages of the upper lamina propria, suggesting induction of TRAP in resident macrophages. Among these cytokines,TNFalpha up-regulated TRAP expression in the RAW 264.7 monocyte/macrophage cell line. In a later phase (day 7) with fulminant colitis, a massive infiltration of macrophages including recruited TRAP+/CCR2+ cells was observed also in the lower part of the lamina propria as well as in the submuscular layer. Additionally, diVerentiated cellular

expression of pro- and mature TRAP also suggest that

mucosal macrophages in the lower part of lamina propria

bordering the sub-mucosa provide a source of replenishment of macrophages situated in the upper lamina propria.

In conclusion, induction of TRAP provides an early sign of macrophage responsiveness in DSS induced colitis

Place, publisher, year, edition, pages
2009. Vol. 132, no 6, p. 599-612
Keywords [en]
DSS, Colitis, Inflammatory bowel disease, Macrophage, Tartrate resistant acid phosphatase, Tumor necrosis factor alpha
National Category
Biological Sciences
Research subject
Biomedical Science
Identifiers
URN: urn:nbn:se:kau:diva-9983OAI: oai:DiVA.org:kau-9983DiVA, id: diva2:493497
Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2017-12-07Bibliographically approved

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CiteExportLink to record
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