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Test av immunhistokemiska markören CK 20 vid differentialdiagnostik av trichoepiteliom och basalcellscancer
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2012 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Trichoepiteliom och basalcellscancer är två olika typer av hudtumörer. Trichoepiteliom är en benign hudtumör och basalcellscancer är en malign hudtumör. De båda tumörerna kan vara svåra att differentiera vid diagnostisering. I trichoepiteliom finns det cytokeratin (CK) 20-positiva merkelceller vilket det inte gör i basalcellscancer. Syftet med studien var att undersöka om immunhistokemisk färgning av CK 20 kan vara till hjälp i rutindiagnostik för att skilja basalcellscancer från trichoepiteliom.  I denna studie användes immunhistokemi (EnVision, Dako, Danmark), med monoklonala antikroppar (FLEX monoclonal antihuman Cytokeratin 20 klon Ks 20.8 ready-to-use kod IR 777 Dako, Danmark), som är riktade mot CK 20. Resultatet visade att 11 av 13 fall med trichoepiteliom innehöll CK 20-positiva merkelceller. Samtliga 12 fall med basalcellscancer saknade CK 20-negativa merkelceller. Av resultatet blev slutsatsen att om det finns CK 20-positiva merkelceller kan detta stödja att det är ett trichoepiteliom om morfologin talar för detta.

Abstract [en]

Trichoepitelioma and basal cell carcinoma are two different types of skin tumors. Trichoepitelioma is a benign skin tumor whereas basal cell carcinoma is a malignant skin tumor. Both tumors can be difficult to differentiate and diagnose. In trichoepitelioma there is cytokeratin (CK) 20-positive merkel cells whereas there is no merkel cell in basal cell carcinoma. The purpose of this study was to investigate whether immunohistochemical staining of CK 20 may be helpful in routine diagnostics to distinguish basal cell carcinoma from trichoepilelioma. In this study, immunohistochemistry (EnVision, Dako, Danmark) has been used, with monoclonal antibodies (FLEX monoclonal antihuman Cytokeratin 20 clone Ks 20.8 ready-to-use code IR 777 Dako, Danmark) directed against CK 20. The results show that in11 of 13 cases with trichoepiteliom the staining for CK 20 was evident. However in all cases with basal cell carcinoma the staining for CK 20 was absent. All 12 cases of basal cell carcinoma were missing CK 20-positive merkel cells it may indicate that, if there is CK 20-positive merkel cells may support that it is a trichoepitelioma if morphology support this conclusion.

Place, publisher, year, edition, pages
2012. , 23 p.
Keyword [sv]
differentialdiagnostik, trichoepiteliom, basalcellscancer
National Category
Biomedical Laboratory Science/Technology
URN: urn:nbn:se:lnu:diva-17223OAI: diva2:488508
External cooperation
Länssjukhuset i Kalmar
Educational program
Biomedical Laboratory Science Programme, 180 credits
Available from: 2012-02-02 Created: 2012-02-01 Last updated: 2012-10-08Bibliographically approved

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