Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression
2004 (English)In: BMC Cancer, ISSN 1471-2407, Vol. 4, no 80Article in journal (Refereed) Published
Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. Results: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. Conclusion: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity.
Place, publisher, year, edition, pages
BioMed Central , 2004. Vol. 4, no 80
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-74455DOI: 10.1186/1471-2407-4-80ISI: 000226525400001OAI: oai:DiVA.org:liu-74455DiVA: diva2:484783