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Co(2+) Selectivity of Thermotoga maritima CorA and Its Inability to Regulate Mg(2+) Homeostasis Present a New Class of CorA Proteins
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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2011 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 286, no 18Article in journal (Refereed) Published
Abstract [en]

CorA is a family of divalent cation transporters ubiquitously present in bacteria and archaea. Although CorA can transport both Mg(2+) and Co(2+) almost equally well, its main role has been suggested to be that of primary Mg(2+) transporter of prokaryotes and hence the regulator of Mg(2+) homeostasis. The reason is that the affinity of CorA for Co(2+) is relatively low and thus considered non-physiological. Here, we show that Thermotoga maritima CorA (TmCorA) is incapable of regulating the Mg(2+) homeostasis and therefore cannot be the primary Mg(2+) transporter of T. maritima. Further, our in vivo experiments confirm that TmCorA is a highly selective Co(2+) transporter, as it selects Co(2+) over Mg(2+) at > 100 times lower concentrations. In addition, we present data that show TmCorA to be extremely thermostable in the presence of Co(2+). Mg(2+) could not stabilize the protein to the same extent, even at high concentrations. We also show that addition of Co(2+), but not Mg(2+), specifically induces structural changes to the protein. Altogether, these data show that TmCorA has the role of being the transporter of Co(2+) but not Mg(2+). The physiological relevance and requirements of Co(2+) in T. maritima is discussed and highlighted. We suggest that CorA may have different roles in different organisms. Such functional diversity is presumably a reflection of minor, but important structural differences within the CorA family that regulate the gating, substrate selection, and transport.

Place, publisher, year, edition, pages
2011. Vol. 286, no 18
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-68126DOI: 10.1074/jbc.M111.222166ISI: 000290022800089OAI: diva2:472175

authorCount :6

Available from: 2012-03-20 Created: 2012-01-03 Last updated: 2013-10-07Bibliographically approved

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