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Genome closure and bioinformatic analysis of the parallel sequenced bacterium Brachyspira intermedia PWS/AT
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Brachyspira species are bacteria that colonize the intestines of some mammalian and avian species with different degrees of pathogenicity. Brachyspira intermedia is a mild pig and bird pathogen with an unknown genomic sequence. In this project, we completed the genome of Brachyspira intermedia PWS/AT and did a comparative genomic analysis between B. intermedia PWS/AT and the already completed genomes of B. hyodysenteriae WA1, B. murdochii 56-150T and B. pilosicoli 95/1000. A table containing 15 classes of unique and shared genes was developed and analyzed in order to gain a better understanding of species-specific traits and clues behind the different degree of pathogenicity. Our result shows that genes are overall poorly annotated and further studies are of great importance for understanding different and shared properties. The largest number of unique features was found in B. intermedia and B. murdochii. B. hyodysenteriae and B. pilosicoli has most likely developed independently towards different biological niches and B. pilosicoli has undergone a major reductive evolution. One plasmid and six prophages were found in B. intermedia, where two of the phages appear to be capable of horizontal gene transfer. Further genome sequencing of more strains will probably increase the understanding of species-specific traits even more.

Place, publisher, year, edition, pages
2011. , 18 p.
Keyword [en]
Brachyspira intermedia, genome closure, comparative genomics, bacteriophages, horizontal gene transfer.
National Category
Engineering and Technology
URN: urn:nbn:se:uu:diva-164335OAI: diva2:467392
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2011-12-19 Created: 2011-12-19 Last updated: 2011-12-19Bibliographically approved

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