Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effects of Adenosine A(2A) and A(2B) Receptor Activation on Signaling Pathways and Cytokine Production in Human Uroepithelial Cells
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2010 (English)In: Pharmacology, ISSN 0031-7012, E-ISSN 1423-0313, Vol. 86, no 3, p. 129-137Article in journal (Refereed) Published
Abstract [en]

Aims: Adenosine A(2A) and A(2B) receptor subtypes have both been implicated in the modulation of inflammation. We examined adenosine A(2A) and A(2B) receptor expression, signaling pathways and the effect of adenosine A(2A) and A(2B) receptor activation on the uropathogenic Escherichia coli (UPEC)-stimulated IL-8 response in human uroepithelial cells (UROtsa). Methods: Receptor expression was examined by RT-PCR and Western blot, and IL-8 production, intracellular cAMP levels and phosphoproteins were measured by ELISA, EIA and multiplex immunoassay, respectively. Results: The adenosine A(1), A(2A) and A(2B) receptor subtypes were detected in UROtsa cells. The adenosine A(2A) receptor agonist CGS 21680 did not stimulate cAMP production but CREB phosphorylation was slightly increased. The adenosine A(2) receptor agonist CPCA induced a pronounced cAMP and CREB response. Furthermore, CGS 21680 but not CPCA decreased ERK 1/2 and STAT3 phosphorylation. UPEC infection stimulated the host IL-8 production but CPCA or CGS 21680 did not affect UPEC-evoked IL-8 production. Conclusions: Our data identified differences in signaling pathways evoked by adenosine A(2A) and A(2B) receptor activation. Activation of the adenosine A(2A) receptor inhibited STAT3 and ERK 1/2 phosphorylation, while the cAMP-CREB pathway was induced by adenosine A(2B) receptor activation. No anti-or proinflammatory effects were found for uroepithelial adenosine A(2A) or A(2B) receptors. Copyright (C) 2010 S. Karger AG, Basel

Place, publisher, year, edition, pages
2010. Vol. 86, no 3, p. 129-137
Keyword [en]
Adenosine receptors, Cyclic AMP, Interleukin-8, Urinary tract infection, Uropathogenic Escherichia coli
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-16182DOI: 10.1159/000317068ISI: 000281224500001Scopus ID: 2-s2.0-77955110191OAI: oai:DiVA.org:lnu-16182DiVA: diva2:466486
Available from: 2011-12-16 Created: 2011-12-16 Last updated: 2017-12-08Bibliographically approved

Open Access in DiVA

fulltext(1243 kB)286 downloads
File information
File name FULLTEXT01.pdfFile size 1243 kBChecksum SHA-512
53c9ebebaaf2194969145e38ca0820ed2ba1e6e1d4033594f3b6da8796ee48d6f246b5afff119da9274b2c5e3ad030361e269501ba7a09cfdcbe93c00ff94a0a
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Säve, SusannePersson, Katarina
By organisation
School of Natural Sciences
In the same journal
Pharmacology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 286 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 53 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf