NK Cell Function and Antibodies Mediating ADCC in HIV-1-Infected Viremic and Controller Patients
2011 (English)In: Viral immunology, ISSN 0882-8245, E-ISSN 1557-8976, Vol. 24, no 5, 359-368 p.Article in journal (Refereed) Published
Natural killer (NK) cells have been suggested to play a protective role in HIV disease progression. One potent effector mechanism of NK cells is antibody-dependent cellular cytotoxicity (ADCC) mediated by antiviral antibodies binding to the Fc gamma RIIIa receptor (CD16) on NK cells. We investigated NK cell-mediated ADCC function and the presence of ADCC antibodies in plasma from 20 HIV-1-infected patients and 10 healthy donors. The HIV-positive patients were divided into two groups: six who controlled viremia for at least 8 y without treatment (controllers), and 14 who were persistently viremic and not currently on treatment. Plasma from both patient groups induced NK cell IFN-gamma expression and degranulation in response to HIV-1 envelope (Env) gp140-protein-coated cells. Patient antibodies mediating ADCC were largely directed towards the Env V3 loop, as identified by a gp140 protein lacking the V3 loop. Interestingly, in two controllers ADCC-mediating antibodies were more broadly directed to other parts of Env. A high viral load in patients correlated with decreased ADCC-mediated cytolysis of gp140-protein-coated target cells. NK cells from both infected patients and healthy donors degranulated efficiently in the presence of antibody-coated HIV-1-infected Jurkat cells. In conclusion, the character of ADCC-mediating antibodies differed in some controllers compared to viremic patients. NK cell ADCC activity is not compromised in HIV-infected patients.
Place, publisher, year, edition, pages
Mary Ann Liebert , 2011. Vol. 24, no 5, 359-368 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-72265DOI: 10.1089/vim.2011.0025ISI: 000296374900002OAI: oai:DiVA.org:liu-72265DiVA: diva2:458802
Funding Agencies|Swedish Research Council||Swedish International Development Cooperation Agency (SIDA)||Swedish Physicians Against AIDS Research Foundation||2011-11-242011-11-242011-12-16