On the analyses of fluorescence depolarisation data in the presence of electronic energy migration: Part I. Theory and general description
2012 (English)In: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 14, 1907-1916 p.Article in journal (Refereed) Published
A new and general procedure is described for a detailed analysis of time-resolved fluorescence depolarisation data in the presence of electronic energy migration. An isotropic ensemble of bifluorophoric molecules (D1-R-D2) has been studied to demonstrate its utility. Intramolecular donor-donor energy migration occurs between the two donor groups (D), which are covalently connected to a rigid linker group (R). These groups undergo restricted reorientational motions with respect to the R group. The analysis of depolarisation data basically involves the search for best-fit parameters which describe the local reorienting motions, the intermolecular D1-D2 distance, as well as the mutual orientations of the donors. For this, the analysis is partly performed in the Fourier domain and the best-fit parameters are determined by using an approach based on a Genetic Algorithm. The energy migration process has been described by using Monte Carlo simulations and an extended Förster theory (EFT). It is found that the EFT provides the least time-consuming computational method. Since one-photon and two-photon excited fluorescence experiments can be applied for energy migration studies, a general and unified theoretical formulation is given.
Place, publisher, year, edition, pages
Cambridge: RSC Publishing, 2012. Vol. 14, 1907-1916 p.
Extended Förster theory, Genetic algorithm, Monte Carlo simulations, two-photon excitation
Physical Chemistry Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:umu:diva-50048DOI: 10.1039/c1cp22483bOAI: oai:DiVA.org:umu-50048DiVA: diva2:458797