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Electrochemical Control of Growth Factor Presentation To Steer Neural Stem Cell Differentiation
Cell and Molecular Biology, Karolinska Institute, Stockholm.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
Cell and Molecular Biology, Karolinska Institute, Stockholm.
Linköping University, Department of Physics, Chemistry and Biology, Surface Physics and Chemistry. Linköping University, The Institute of Technology.
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2011 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 50, no 52, 12529-12533 p.Article in journal (Refereed) Published
Abstract [en]

Graphical Abstract

Let it grow: The conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was synthesized with heparin as the counterion to form a cell culture substrate. The surface of PEDOT:heparin in the neutral state associated biologically active growth factors (see picture). Electrochemical in situ oxidation of PEDOT during live cell culture decreased the bioavailability of the growth factor and created an exact onset of neural stem cell differentiation.

Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 2011. Vol. 50, no 52, 12529-12533 p.
National Category
Polymer Chemistry Cell Biology
URN: urn:nbn:se:liu:diva-72171DOI: 10.1002/anie.201103728ISI: 000298332700018PubMedID: 22057546OAI: diva2:457903
funding agencies|Swedish Research Council (VR)||Swedish Foundation for Strategic Research (SSF; the OBOE center)||Karolinska Institute||VR||Onnesjo foundation||Linkoping University||Available from: 2011-11-21 Created: 2011-11-21 Last updated: 2017-12-08
In thesis
1. Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces
Open this publication in new window or tab >>Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In the field of bioelectronics various electronic materials and devices are used in combination with biological systems in order to create novel applications within cell biology and medicine. A famous example of a successful bioelectronics application is the pacemaker. Metals are the most common electrical conductors, whereas polymers are generally considered being insulators. However, in the late 1970s it was shown that a special class of polymers with conjugated double bonds, could in fact, after some chemical modifications, conduct electricity. This was the start of the research field known as conducting polymers, and then later on organic electronics, a research area that has grown rapidly during the last decades. Conjugated polymers are also suitable to interact and interface with cells and tissues, as they are generally soft, flexible and biocompatible. In addition, their chemical properties can be tailor-made through synthesis to fit biological requirements and functions. During the last years applications using organic bioelectronics have become numerous.

This thesis describes applications based on different conjugated polymers aiming to stimulate and control cell cultures. When culturing cells it is of interest to be able to control events such as adhesion, spreading, proliferation, differentiation and detachment. First, the impact of different polymer compositions and redox states on the adhesion of bacteria and subsequent biofilm formation was investigated. Similar polymer electrodes were also used to steer differentiation of neural stem cells, through changes in the surface exposure of a relevant biomolecule. Controlled delivery of molecules was achieved by coating nanoporous membranes with polymers that swell and contract when changing the redox state. Finally, electronic control over cell detachment using a water-soluble polymer was achieved. When applying a positive potential to this polymer, it swells, cracks and finally detaches, taking the cells that was cultured on top along with it. Together, the work and results presented in this thesis demonstrate a versatile conjugated polymer technology to achieve electronic control of the different growth stages of cell cultures as well as cellular functions.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2014. 64 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1594
National Category
Natural Sciences
urn:nbn:se:liu:diva-106254 (URN)10.3384/diss.diva-106254 (DOI)978-91-7519-340-3 (ISBN)
Public defence
2014-05-23, K2, Kåkenhus, Campus Valla, Linköpings universitet, Linköping, 10:15 (English)
Available from: 2014-04-30 Created: 2014-04-30 Last updated: 2017-02-03Bibliographically approved

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