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Optimization of proximity ligationassay based Western blotting
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Many of today’s methods for the detection of biomolecules suffer from a high limit ofdetection due to poor signal generation upon recognition of target. By applying andoptimizing proximity ligation assay (PLA) in Western blotting (WB), the limit of detectionhas been lowered down to the picomolar range. In this report I have optimized the differentparameters that affect the signal generation and explored possibilities to increase the ease ofuse, by merging protocol steps and performing signal generating reactions at roomtemperature.

Place, publisher, year, edition, pages
2011. , 30 p.
, UPTEC X, 11035
Keyword [en]
Western blotting, proximity ligation assay, signal amplification, specificity, optimization
National Category
Industrial Biotechnology
URN: urn:nbn:se:uu:diva-161849OAI: diva2:457758
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2011-11-23 Created: 2011-11-19 Last updated: 2011-11-23Bibliographically approved

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fulltext(1557 kB)634 downloads
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Type fulltextMimetype application/pdf

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Johansson, Johan
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Biology Education Centre
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