Optimization of proximity ligationassay based Western blotting
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Many of today’s methods for the detection of biomolecules suffer from a high limit ofdetection due to poor signal generation upon recognition of target. By applying andoptimizing proximity ligation assay (PLA) in Western blotting (WB), the limit of detectionhas been lowered down to the picomolar range. In this report I have optimized the differentparameters that affect the signal generation and explored possibilities to increase the ease ofuse, by merging protocol steps and performing signal generating reactions at roomtemperature.
Place, publisher, year, edition, pages
2011. , 30 p.
, UPTEC X, 11035
Western blotting, proximity ligation assay, signal amplification, specificity, optimization
IdentifiersURN: urn:nbn:se:uu:diva-161849OAI: oai:DiVA.org:uu-161849DiVA: diva2:457758
Molecular Biotechnology Engineering Programme
Hagner-McWhirter, Åsa, Postdoc