Development of novel multiplexed systems for in situ PLA
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
The in situ proximity ligation assay (in situ PLA) is an immunoassay that enables directvisualisation of single protein targets or protein interactions in cell or tissue samples. This project revolves around designing and introducing several novel multiplexable components tobe used in conjunction with Olink Bioscience's Duolink product line. In this report, a novel in silico approach to DNA oligomer interaction design is presented. Using this in silico method, a multiplexed system of DNA oligomers has been designed andevaluated using in situ PLA and fluorescence microscopy.
Place, publisher, year, edition, pages
2011. , 28 p.
Proximity ligation assay, PLA, Multiplex, Immunoassay, Rolling circle amplification, RCA, in situ, DNA sequence design
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-161126OAI: oai:DiVA.org:uu-161126DiVA: diva2:454763
Molecular Biotechnology Engineering Programme
Gullberg, Mats, PhD
Söderberg, Ola, Docent