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Development of novel multiplexed systems for in situ PLA
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The in situ proximity ligation assay (in situ PLA) is an immunoassay that enables directvisualisation of single protein targets or protein interactions in cell or tissue samples. This project revolves around designing and introducing several novel multiplexable components tobe used in conjunction with Olink Bioscience's Duolink product line. In this report, a novel in silico approach to DNA oligomer interaction design is presented. Using this in silico method, a multiplexed system of DNA oligomers has been designed andevaluated using in situ PLA and fluorescence microscopy.

Place, publisher, year, edition, pages
2011. , 28 p.
Keyword [en]
Proximity ligation assay, PLA, Multiplex, Immunoassay, Rolling circle amplification, RCA, in situ, DNA sequence design
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-161126OAI: oai:DiVA.org:uu-161126DiVA: diva2:454763
Educational program
Molecular Biotechnology Engineering Programme
Uppsok
Technology
Supervisors
Examiners
Available from: 2011-11-08 Created: 2011-11-07 Last updated: 2011-11-08Bibliographically approved

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Development of novel multiplexed systems for in situ PLA(1178 kB)450 downloads
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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
  • de-DE
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  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
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Output format
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