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Expression pattern of GPI-anchored non-specific lipid transfer proteins in Physcomitrella patens
Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics .
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Abstract [en]

During the water-to-land transition, that occurred approximately 450 MYA, novel habitats wererevealed to the emerging plants. This terrestrial habitat was a harsh environment compared to theaquatic, with shifting substrate content, irregular supply of water, damaging UV-radiation andrapid fluctuating temperatures. Non-specific lipid transfer proteins (nsLTP) are today only foundin the land living plants and not in the green algae. This suggests that these genes might haveevolved to help the plants cope with the stressful conditions. In this study the expression patternhas been analysed of the nsLTPs in the moss Physcomitrella patens during the possible conditionsthat raised during the water-to-land transition. The moss was exposed to salt, UV-B, drought, copper, cold and osmotic stress. Quantitative real-time PCR was used to analyse the transcription levels. I found that six genes were upregulated during either cold, dehydration or UV-B stress. This suggest that these genes are involved in the plant defense against these abiotic stresse

Place, publisher, year, edition, pages
2011. , 16 p.
Keyword [en]
Abiotic stress, lipid transfer proteins, moss, nsLTP, Physcomitrella patens, qRT-PC
National Category
URN: urn:nbn:se:liu:diva-71702ISRN: LiTH-IFM- Ex-- 11/2427--SEOAI: diva2:453083
Life Earth Science
Available from: 2011-11-01 Created: 2011-11-01 Last updated: 2011-11-01Bibliographically approved

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