The HER2-binding Affibody Molecule (ZHER2:342)2 Increases Radiosensitivity in SKBR-3 cells
2012 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 7, no 11, e49579- p.Article in journal (Refereed) Published
We have previously shown that the HER2-specific affibody molecule (ZHER2:342)2 inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (ZHER2:342)2 sensitizes the HER2-overexpressing cell line SKBR-3 to ionizing radiation. The survival after exposure to (ZHER2:342)2 and 8 Gy (S8Gy 0.006) was decreased by a factor of 4 compared to the untreated (S8Gy 0.023). The low HER2-expressing cell line MCF-7 was more radiosensitive than SKBR-3 but did not respond to (ZHER2:342)2. Treatment by (ZHER2:342)2 strongly increased the levels of dimerized and phosphorylated HER2 already after 5 minutes of stimulation. The monomeric ZHER2:342 does not seem to be able to induce receptor phosphorylation and dimerization or sensitize cells to irradiation.
Place, publisher, year, edition, pages
2012. Vol. 7, no 11, e49579- p.
HER2, Affibody molecule, receptor dimerization, receptor phosphorylation
Medical and Health Sciences
Research subject Biomedical Radiation Science
IdentifiersURN: urn:nbn:se:uu:diva-160169DOI: 10.1371/journal.pone.0049579ISI: 000311151900167PubMedID: 23166716OAI: oai:DiVA.org:uu-160169DiVA: diva2:449056