Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Conjugated Polymer Surface Switches for Active Control
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polymers have been found useful in a wide range of applications such as sensors, electrochemical transistors, solar cells, and printed electronics due to their mechanical, optical and electronic properties. An amazing research field has grown during the last three decades since the discovery of conducting polymers in 1976. Since the materials can be made from solutions, different processing methods such as spin coating and vapor phase polymerization can be used to coat a huge variety of substrates. The choice of method depends mainly on monomer solubility and kind of substrate to be coated. During the synthesis the polymers can be chemically modified to tailor their functionalities. Due to this variability in materials and the processability, electronics can be achieved on unconventional substrates such as flexible plastic foils and cell culturing dishes. As a contrast to inorganic, usually metallic materials, conducting polymers are built up from organic compounds in a molecular structure with soft mechanical properties that have shown to be a benefit in combination with biology, ranging from interactions with cells to interactions with advanced biological species such as tissues. This combination of research fields and the possible applications are merged within the field of organic bioelectronics.

The primary purpose of this thesis is to give a background to organic electronics in general and how electrochemical devices can be processed and developed for biological applications in particular. An organic electronic surface switch is introduced to control cell adhesion and proliferation as well as an electrochemical transistor to spatially tune the cell adhesion along an electrochemical gradient. To mimic a more natural cell environment a three dimensional fiber substrate was used to design an electronically active matrix to promote nerve cell adhesion and communication. By combining standard microfabrication techniques and conjugated polymers desired patterns of electroactive polymer were created to enable active regulation of cell populations and their extracellular environment at high spatial resolution. Finally, a brief look into future challenges will also be presented.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. , 52 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1398
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-71361ISBN: 978-91-7393-063-5 (print)OAI: oai:DiVA.org:liu-71361DiVA: diva2:447707
Public defence
2011-10-21, K3, Kåkenhus, Campus Norrköping, Linköpings universitet, Norrköping, 13:15 (English)
Opponent
Supervisors
Available from: 2011-10-13 Created: 2011-10-13 Last updated: 2017-02-03Bibliographically approved
List of papers
1. Control of Neural Stem Cell Adhesion and Density by an Electronic Polymer Surface Switch
Open this publication in new window or tab >>Control of Neural Stem Cell Adhesion and Density by an Electronic Polymer Surface Switch
Show others...
2008 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 24, no 24, 14133-14138 p.Article in journal (Refereed) Published
Abstract [en]

Adhesion is an essential parameter for stem cells. It regulates the overall cell density along the carrying surface, which further dictates the differentiation scheme of stem cells toward a more matured and specified population as well as tissue. Electronic control of the seeding density of neural stem cells (c17.2) is here reported. Thin electrode films of poly(3,4-ethylenedioxythiophene) (PEDOT):Tosylate were manufactured along the floor of cell growth dishes. As the oxidation state of the conjugated polymer electrodes was controlled, the seeding density could be varied by a factor of 2. Along the oxidized PEDOT:Tosylate-electrodes, a relatively lower density of, and less tightly bonded, human serum albumin (HSA) was observed as compared to reduced electrodes. We found that this favors adhesion of the specific stem cells studied. Surface analysis experiments, such as photoelectron spectroscopy, and water contact angle measurements, were carried out to investigate the mechanisms responsible for the electronic control of the seeding density of the c17.2 neural stem cells. Further, our findings may provide an opening for electronic control of stem cell differentiation.

National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-16247 (URN)10.1021/la8028337 (DOI)
Available from: 2009-01-12 Created: 2009-01-09 Last updated: 2017-12-14
2. Electrochemical modulation of epithelia formation using conducting polymers
Open this publication in new window or tab >>Electrochemical modulation of epithelia formation using conducting polymers
Show others...
2009 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 30, no 31, 6257-6264 p.Article in journal (Refereed) Published
Abstract [en]

Conducting polymers are soft, flexible materials, exhibiting material properties that can be reversibly changed by electrochemically altering the redox state. Surface chemistry is an important determinant for the molecular events of cell adhesion. Therefore, we analyzed whether the redox state of the conducting polymer PEDOT:Tosylate can be used to control epithelial cell adhesion and proliferation. A functionalized cell culture dish comprising two adjacent electrode surfaces was developed. Upon electronic addressing, reduced and oxidized surfaces are created within the same device. Simultaneous analysis of how a homogenous epithelial MDCK cell population responded to the electrodes revealed distinct surface-specific differences. Presentation of functional fibronectin on the reduced electrode promoted focal adhesion formation, involving αvβ3 integrin, cell proliferation, and ensuing formation of polarized monolayers. In contrast, the oxidized surface harbored only few cells with deranged morphology showing no indication of proliferation. This stems from the altered fibronectin conformation, induced by the different surface chemistry of the PEDOT:Tosylate electrode in the oxidized state. Our results demonstrate a novel use of PEDOT:Tosylate as a cell-hosting material in multiple-electrode systems, where cell adhesion and proliferation can be controlled by electrochemical modulation of surface properties.

Keyword
Actin; Cell adhesion; Electroactive polymer; Epithelial cell; Fibronectin; RGD peptide
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-20545 (URN)10.1016/j.biomaterials.2009.07.059 (DOI)
Note
Original Publication: Karl Svennersten, Maria H. Bolin, Edwin W.H. Jager, Magnus Berggren and Agneta Richter-Dahlfors, Electrochemical modulation of epithelia formation using conducting polymers, 2009, Biomaterials, (30), 31, 6257-6264. http://dx.doi.org/10.1016/j.biomaterials.2009.07.059 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/ Available from: 2009-09-11 Created: 2009-09-11 Last updated: 2017-12-13
3. Active Control of Epithelial Cell-Density Gradients Grown Along the Channel of an Organic Electrochemical Transistor
Open this publication in new window or tab >>Active Control of Epithelial Cell-Density Gradients Grown Along the Channel of an Organic Electrochemical Transistor
Show others...
2009 (English)In: ADVANCED MATERIALS, ISSN 0935-9648, Vol. 21, no 43, 4379- p.Article in journal (Refereed) Published
Abstract [en]

Complex patterning of the extracellular matrix, cells, and tissues under in situ electronic control is the aim of the technique presented here. The distribution of epithelial cells along the channel of an organic electrochemical transistor is shown to be actively controlled by the gate and drain voltages, as electrochemical gradients are formed along the transistor channel when the device is biased.

National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-52838 (URN)10.1002/adma.200901191 (DOI)
Available from: 2010-01-12 Created: 2010-01-12 Last updated: 2017-02-03
4. Nano-fiber scaffold electrodes based on PEDOT for cell stimulation
Open this publication in new window or tab >>Nano-fiber scaffold electrodes based on PEDOT for cell stimulation
Show others...
2009 (English)In: SENSORS AND ACTUATORS B-CHEMICAL, ISSN 0925-4005, Vol. 142, no 2, 451-456 p.Article in journal (Refereed) Published
Abstract [en]

Electronically conductive and electrochemically active 3D-scaffolds based on electrospun poly(ethylene terephthalate) (PET) nano-fibers are reported. Vapour phase polymerization was employed to achieve an uniform and conformal coating of poly(3,4-ethylenedioxythiophene) doped with tosylate (PEDOT:tosylate) on the nano-fibers. The PEDOT coatings had a large impact on the wettability, turning the hydrophobic PET fibers super-hydrophilic. SH-SY5Y neuroblastoma cells were grown on the PEDOT coated fibers. The SH-SY5Y cells adhered well and showed healthy morphology. These electrically active scaffolds were used to induce Ca2+ signalling in SH-SY5Y neuroblastoma cells. PEDOT:tosylate coated nano-fibers represent a class of 3D host environments that combines excellent adhesion and proliferation for neuronal cells with the possibility to regulate their signalling.

Keyword
Poly(ethylenedioxythiophene) (PEDOT), Electrospinning, Poly(ethylene terephthalate) (PET), Cell stimulation, SH-SY5Y neuroblastoma cells, Nano-fibers
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-52395 (URN)10.1016/j.snb.2009.04.062 (DOI)
Note
Original Publication: Maria Bolin, Karl Svennersten, Xiangjun Wang, Ioannis S Chronakis, Agneta Richter-Dahlfors, Edwin Jager and Magnus Berggren, Nano-fiber scaffold electrodes based on PEDOT for cell stimulation, 2009, SENSORS AND ACTUATORS B-CHEMICAL, (142), 2, 451-456. http://dx.doi.org/10.1016/j.snb.2009.04.062 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/ Available from: 2010-01-11 Created: 2009-12-18 Last updated: 2017-02-03
5. Electroactive Control of Platelet Adhesion to Conducting Polymer Micropatterns
Open this publication in new window or tab >>Electroactive Control of Platelet Adhesion to Conducting Polymer Micropatterns
Show others...
(English)Manuscript (preprint) (Other academic)
Abstract [en]

In this work, we report a method to fabricate addressable micropatterns of electroactive surfaces based on the conducting polymer poly-(3, 4-ethylenedioxytiophene) (PEDOT:Tos) to gain dynamic control over the spatial distribution of platelets, in vitro. Utilizing thin film processing and microfabrication techniques desired patterns down to the scale of  individual cells, were achieved to enable active regulation of cell  populations and their extracellular environment at high spatial resolution.Upon electronic addressing, both reduced and oxidized surfaces were created within the same device. The changes of the electrochemical state of PEDOT results in a reversible modification of the surface properties of the material. This surface modulation dictates the conformation and/or orientation, rather than the concentration, of surface proteins, thus indirectly regulating cell adhesion. The chemistry, texture, charge, and softness of fiacrtiial cell-hosting surfaces are parameters known to affect the binding characteristics and orientation of the extracellular proteins, thus dictating adhesion, spreading, migration, and proliferation of cells.

National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-71360 (URN)
Available from: 2011-10-13 Created: 2011-10-13 Last updated: 2017-02-03Bibliographically approved

Open Access in DiVA

Conjugated Polymer Surface Switches for Active Control(1406 kB)1954 downloads
File information
File name FULLTEXT01.pdfFile size 1406 kBChecksum SHA-512
6bd4da98e317e97e0c656965765a786bfaf03a85023c4cacd25050ceebd97e77881ae187cb8a022e420ea2c5d0865cf869e859e45b002aaa288d4e70554e953c
Type fulltextMimetype application/pdf
omslag(206 kB)106 downloads
File information
File name COVER01.pdfFile size 206 kBChecksum SHA-512
53b01faecdcda2ddca99fd5924fd2d14f1096e0be87224cfa39e31f17c9d41011a0822afc4b213bce2b14a07471119b9a41c5d7bf7948fd482a63378dd86b6cc
Type coverMimetype application/pdf

Search in DiVA

By author/editor
Bolin, Maria
By organisation
Department of Science and TechnologyThe Institute of Technology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 1954 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 9280 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf