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Sample preparation of 8-hydroxy-2’-deoxyguanosine with solid phase extraction methodology based on molecular imprinting polymers and conventional silica based phases
Linköping University, Department of Physics, Chemistry and Biology.
2011 (English)Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
Abstract [en]

The aim of this study was to develop methods for sample preparation for 8-OHdG in blood plasma samples with different solid phase extraction techniques using HPLC with an elec- trochemical detector. The solid phase extraction cartridges used were Chromabond® C18, Oasis® MAX, and three types of SupelMIPTM cartridges for chloramphenicol, riboflavin, and nitroimidazoles. The SupelMIPTM cartridges are based on molecularly imprinted polymers- technique. The separation of 8-OHdG in samples extracted from blood plasma was carried out with a Thermo Quest Hypersil Division ODS column (250 mm × 4 mm, 3μm I.D.) and methanol:buffer (10:90, v/v) as mobile phase. Recovery and selectivity was studied for the different solid phase extraction methods. The highest recovery was obtained using the Chromabond C18 cartridge with a recovery of 92%, and CV coefficient 9.5% (n = 4). 8-OHdG could not be extracted on MIP-cartridges for chloramphenicol or riboflavin, but was retained on MIP columns for nitroimidazoles, and the highest recovery was 49%.

Place, publisher, year, edition, pages
2011. , 33 p.
Keyword [en]
SPE, solid phase extraction, MIP, molecularly imprinted polymers, HPLC
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-71325ISRN: LITH-IFM-G--11/2468--SEOAI: oai:DiVA.org:liu-71325DiVA: diva2:447420
Subject / course
Chemistry, Other
Presentation
2011-06-10, Dahlén, Linköping, 10:15 (Swedish)
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2011-10-13 Created: 2011-10-11 Last updated: 2011-10-13Bibliographically approved

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Analytical Chemistry

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CiteExportLink to record
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