Sample preparation of 8-hydroxy-2’-deoxyguanosine with solid phase extraction methodology based on molecular imprinting polymers and conventional silica based phases
Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
The aim of this study was to develop methods for sample preparation for 8-OHdG in blood plasma samples with different solid phase extraction techniques using HPLC with an elec- trochemical detector. The solid phase extraction cartridges used were Chromabond® C18, Oasis® MAX, and three types of SupelMIPTM cartridges for chloramphenicol, riboflavin, and nitroimidazoles. The SupelMIPTM cartridges are based on molecularly imprinted polymers- technique. The separation of 8-OHdG in samples extracted from blood plasma was carried out with a Thermo Quest Hypersil Division ODS column (250 mm × 4 mm, 3μm I.D.) and methanol:buffer (10:90, v/v) as mobile phase. Recovery and selectivity was studied for the different solid phase extraction methods. The highest recovery was obtained using the Chromabond C18 cartridge with a recovery of 92%, and CV coefficient 9.5% (n = 4). 8-OHdG could not be extracted on MIP-cartridges for chloramphenicol or riboflavin, but was retained on MIP columns for nitroimidazoles, and the highest recovery was 49%.
Place, publisher, year, edition, pages
2011. , 33 p.
SPE, solid phase extraction, MIP, molecularly imprinted polymers, HPLC
IdentifiersURN: urn:nbn:se:liu:diva-71325ISRN: LITH-IFM-G--11/2468--SEOAI: oai:DiVA.org:liu-71325DiVA: diva2:447420
Subject / course
2011-06-10, Dahlén, Linköping, 10:15 (Swedish)
UppsokPhysics, Chemistry, Mathematics
Svensson, Stefan, Dr.