Change search
ReferencesLink to record
Permanent link

Direct link
Comparison between different freezing and thawing methods for human spermatozoa
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2011 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Preservation of cells and tissues by freezing at temperatures below 70°C has led to new possibilities for the storage of germ cells for fertility preservation. During the freezing process problems might occur, the greatest being ice crystallization which can cause membrane destruction and thus cell death. To minimize this risk, solutions that reduce the freezing point can be added to reduce crystallization and increase survival rates. These solutions are called cryoprotectants. The best method for freezing is still not known.The aim of this study was to analyze the effect of various parameters on the survival rate of human semen frozen with liquid nitrogen. The parameters investigated were thawing method (incubator or water bath) and container choice (straw or ampoule). In addition, two different cryoprotectants were tested.The method used was the instruction for preservation with Sperm CryoProtec™ II from Nidacon. In total 16 samples were collected for the first test and 13 samples for the second test. Sperm concentration and motility was measured.There seem to be no significant differences depending on container choice or thawing method leading to the conclusion that the most cost effective method of storage and thawing may be used. A small but significant difference was found in survival after thawing dependent on cryoprotectant p=0.041. However the study sample was limited and further studies might be of value.

Place, publisher, year, edition, pages
2011. , 14 p.
Keyword [en]
cryopreservation, sperm, ice crystallization, gradient wash, ampoule
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-158842OAI: diva2:441666
Educational program
Biomedicinska analytikerprogrammet
Available from: 2011-10-10 Created: 2011-09-17 Last updated: 2011-10-10Bibliographically approved

Open Access in DiVA

fulltext(614 kB)359 downloads
File information
File name FULLTEXT01.pdfFile size 614 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Department of Medical Biochemistry and Microbiology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 359 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 470 hits
ReferencesLink to record
Permanent link

Direct link