Change search
ReferencesLink to record
Permanent link

Direct link
Gene identification in the encystation pathway of the Dictyostelid Polysphondylium pallidum
Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Abstract [en]

Encystation of unicellular organisms is of considerable medical relevance since cysts are encapsulated byresilient cell walls, rendering them resistant to biocides and immune clearance. This survival strategymakes it complicated to produce effective treatment of diseases caused by many protozoan pathogens,e.g. species of Acanthamoeba which causes fatal granulomatous amebic encephalitis (GAE) and keratitis.Due to genetic limitations in most protists, the machinery of encystation is so far little understood.However, the signalling pathways can be investigated in the non-pathogenic social amoebas, Dictyostelia.In this master’s project, five genes in Polysphondylium pallidum were investigated, which might beinvolved in encystation. Research has demonstrated a relationship between encystation and the cyclicadenosine monophosphate (cAMP) signalling pathways in Dictyostelia spore formation. This indicates thatcysts are ancestral to spores, and hence are the sporulation genes: pkaC, yakA, regA, cudA and srfAselected for this study. The genes were individually knocked-out by a standard homologous recombinationapproach and the genes’ contribution to encystation was determined. Five knock-out constructs werecompleted and a preliminary analysis of the role of the intracellular cAMP phosphodiesterase RegA inPolysphondylium pallidum encystation process was performed.

Place, publisher, year, edition, pages
2011. , 63 p.
Keyword [en]
Encystation, Polysphondylium pallidum, RegA, pLox-NeoI
National Category
Chemical Sciences
URN: urn:nbn:se:liu:diva-70458ISRN: LITH-IFM-A-EX--11/2484--SEOAI: diva2:439744
Subject / course
Available from: 2011-09-12 Created: 2011-09-08 Last updated: 2011-09-12Bibliographically approved

Open Access in DiVA

fulltext(2285 kB)395 downloads
File information
File name FULLTEXT01.pdfFile size 2285 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 395 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 116 hits
ReferencesLink to record
Permanent link

Direct link