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c Cytochromes as Electron Carriers in Microbial Chlorate Respiration
Karlstad University, Faculty of Technology and Science, Department of Chemistry and Biomedical Sciences. (Kloratgruppen)
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Microbial respiration of oxochlorates is important for the biotreatment of effluents from industries where oxochlorates are produced or handled. Several bacterial species are capable to use perchlorate and/or chlorate as an alternative electron acceptor in absence of oxygen. The present study deals with the electron transport from the membrane-bound components to the periplasmic chlorate reductase, in the gram-negative bacterium Ideonella dechloratans. Both chlorate reductase and the terminal oxidase of I. dechloratans were found to utilize soluble c cytochromes as electron donors. For further investigation, two major heme-containing components were purified and characterized. The most abundant was a 9 kDa c-type cytochrome (class I), denoted cytochrome c-Id1. This protein was shown to serve as electron donor for both chlorate reductase, and for a terminal oxidase. The other major component was a 55 kDa homotetrameric cytochrome c', (class II). A function for this cytochrome could not be demonstrated but it does not appear to serve as electron donor to chlorate reductase. A gene predicted to encode a soluble c cytochrome was found in close proximity to the gene cluster for chlorate reduction. The predicted sequence did not match any of the cytochromes discussed above. The gene was cloned and expressed heterologously, and the resulting protein was investigated as a candidate electron donor for chlorate reductase. Electron transfer from this protein could not be demonstrated, suggesting that the gene product does not serve as immediate electron donor for chlorate reductase.

Place, publisher, year, edition, pages
Karlstad: Karlstad University , 2011. , 48 p.
Series
Karlstad University Studies, ISSN 1403-8099 ; 2011:40
Keyword [en]
c cytochrome, chlorate reduction, electron transport
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-8038ISBN: 978-91-7063-375-1 (print)OAI: oai:DiVA.org:kau-8038DiVA: diva2:433846
Public defence
2011-09-30, Andersalen, 11D 121, Karlstads universitet, Karlstad, 10:15 (Swedish)
Opponent
Supervisors
Available from: 2011-09-06 Created: 2011-08-11 Last updated: 2011-11-14Bibliographically approved
List of papers
1. Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
Open this publication in new window or tab >>Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
2009 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 75, 2439-2445 p.Article in journal (Refereed) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-2804 (URN)10.1128/AEM.01325-08 (DOI)
Note
Artikelns titel i Anna Smedja Bäcklunds licentiatuppsats (2009): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans" Artikelns titel i Jan Bohlins doktorsavhandling (2008): "Periplasmic c cytochromes coupled to chlorate reduction in Ideonella dechloratans"Available from: 2008-10-08 Created: 2008-10-08 Last updated: 2017-12-11Bibliographically approved
2. Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
Open this publication in new window or tab >>Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
Show others...
2010 (English)In: Microbiology Research, ISSN 0944-5013, E-ISSN 1618-0623, Vol. 165, 450-457 p.Article in journal (Refereed) Published
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8162 (URN)10.1016/j.micres.2009.10.003 (DOI)000282633000002 ()20015627 (PubMedID)
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2017-12-08Bibliographically approved
3. Purification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans
Open this publication in new window or tab >>Purification and characterization of a soluble cytochrome c capable of delivering electrons to chlorate reductase in Ideonella dechloratans
2011 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 321, 115-120 p.Article in journal (Refereed) Published
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8163 (URN)10.1111/j.1574-6968.2011.02321.x (DOI)000292741300005 ()
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2017-12-08Bibliographically approved
4. Purification and characterization of a cytochrome c' from the chlorate respiring bacterium Ideonella dechloratans
Open this publication in new window or tab >>Purification and characterization of a cytochrome c' from the chlorate respiring bacterium Ideonella dechloratans
(English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kau:diva-8164 (URN)
Available from: 2011-09-06 Created: 2011-09-06 Last updated: 2011-11-02Bibliographically approved

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