Change search
ReferencesLink to record
Permanent link

Direct link
Statistical Analysis of Quantitative PCR Data
Norwegian University of Science and Technology, Faculty of Information Technology, Mathematics and Electrical Engineering, Department of Mathematical Sciences.
2011 (English)MasteroppgaveStudent thesis
Abstract [en]
This thesis seeks to develop a better understanding of the analysis of gene expression to find the amount of transcript in a sample. The mainstream method used is called Polymerase Chain Reaction (PCR) and it exploits the DNA's ability to replicate. The comparative CT method estimate the starting fluorescence level f0 by assuming constant amplification in each PCR cycle, and it uses the fluorescence level which has risen above a certain threshold. We present a generalization of this method, where different threshold values can be used. The main aim of this thesis is to evaluate a new method called the Enzymological method. It estimates f0 by considering a cycle dependent amplification and uses a larger part of the fluorescence curves, than the two CT methods. All methods are tested on dilution series, where the dilution factors are known. In one of the datasets studied, the Clusterin dilution-dataset, we get better estimates from the Enzymological method compared to the two CT methods.
Place, publisher, year, edition, pages
Institutt for matematiske fag , 2011. , 98 p.
Keyword [no]
ntnudaim:6159, MTFYMA fysikk og matematikk, Industriell matematikk
URN: urn:nbn:no:ntnu:diva-13094Local ID: ntnudaim:6159OAI: diva2:432285
Available from: 2011-08-02 Created: 2011-08-02

Open Access in DiVA

fulltext(1706 kB)367 downloads
File information
File name FULLTEXT01.pdfFile size 1706 kBChecksum SHA-512
Type fulltextMimetype application/pdf
cover(47 kB)36 downloads
File information
File name COVER01.pdfFile size 47 kBChecksum SHA-512
Type coverMimetype application/pdf

By organisation
Department of Mathematical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 367 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 50 hits
ReferencesLink to record
Permanent link

Direct link