Change search
ReferencesLink to record
Permanent link

Direct link
Restriction in the membrane diffusion over the dividing septum of Escherichia Coli cells measured by  Flourescence Recovery After Photobleaching
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

This diploma thesis is a follow up work of a previous paper by Johan Strömqvist et. al. [1] In their article they presented a novel method on how to determine the Z-ring radius and the contraction in dividing

Escherichia coli. This was done by fluorescent recovery after photobleaching (FRAP) measurements in the cytosol of E.coli cells transfected to express the Enhanced Green Fluorescent Protein (EGFP). The E.coli cell was irreversibly bleached on one side of an already visible invagination in the midcell, and then the fluorescence recovery was followed in time. Since the fluorescence recovery depends on the cross sectional area of the septum that information could be used to derive a mathematical expression to estimate the septal radius of the cell. In this thesis the goal was to, along with cytosolic recovery, examine the recovery after a bleaching pulse in the fluorescent labeled cell membrane. Furthermore it also set out to investigate how the cytosolic fluorescence recovery of EGFP was or could be correlated to the fluorescence recovery of dyes in the cell membranes of the E.coli.

Place, publisher, year, edition, pages
2011. , 45 p.
Trita-FYS, ISSN 0280-316X ; 2
National Category
Physical Sciences
URN: urn:nbn:se:kth:diva-36750OAI: diva2:430996
Educational program
Master of Science in Engineering -Engineering Physics
Physics, Chemistry, Mathematics
Available from: 2011-08-11 Created: 2011-07-14 Last updated: 2011-10-18Bibliographically approved

Open Access in DiVA

fulltext(574 kB)213 downloads
File information
File name FULLTEXT01.pdfFile size 574 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Experimental Biomolecular Physics
Physical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 213 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 78 hits
ReferencesLink to record
Permanent link

Direct link