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Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The number of monoclonal antibodies approved for therapeutic use has increased rapidlyover the last decade. As a consequence, precise and robust kinetic characterization techniquesare crucial in order to select the best suitable candidates. A kinetic characterization methodwas developed in Gyrolab with automated sample transfers. The characterization wasperformed in solution in a mixing CD, containing an integrated nanoliter mixing chamberwith affinity binding columns. Association rate constants were determined for four anti-TSHantibodies with values ranging from 3x105 M-1s-1 to 10x105 M-1s-1. The antibodies wereranked according to kass. Reproducibility

Place, publisher, year, edition, pages
2011. , 53 p.
UPTEC X, 11 029
Keyword [en]
Affinity, association rate constant, Bioaffy, characterization, Gyrolab, immunoassay, kinetics, microfluidics, monoclonal antibody, screening
National Category
Industrial Biotechnology Medical Laboratory and Measurements Technologies Biocatalysis and Enzyme Technology Immunology
URN: urn:nbn:se:uu:diva-155575OAI: diva2:426828
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2011-06-27 Created: 2011-06-27 Last updated: 2011-06-27Bibliographically approved

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Biology Education Centre
Industrial BiotechnologyMedical Laboratory and Measurements TechnologiesBiocatalysis and Enzyme TechnologyImmunology

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