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Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The number of monoclonal antibodies approved for therapeutic use has increased rapidlyover the last decade. As a consequence, precise and robust kinetic characterization techniquesare crucial in order to select the best suitable candidates. A kinetic characterization methodwas developed in Gyrolab with automated sample transfers. The characterization wasperformed in solution in a mixing CD, containing an integrated nanoliter mixing chamberwith affinity binding columns. Association rate constants were determined for four anti-TSHantibodies with values ranging from 3x105 M-1s-1 to 10x105 M-1s-1. The antibodies wereranked according to kass. Reproducibility

Place, publisher, year, edition, pages
2011. , 53 p.
Series
UPTEC X, 11 029
Keyword [en]
Affinity, association rate constant, Bioaffy, characterization, Gyrolab, immunoassay, kinetics, microfluidics, monoclonal antibody, screening
National Category
Industrial Biotechnology Medical Laboratory and Measurements Technologies Biocatalysis and Enzyme Technology Immunology
Identifiers
URN: urn:nbn:se:uu:diva-155575OAI: oai:DiVA.org:uu-155575DiVA: diva2:426828
Educational program
Molecular Biotechnology Engineering Programme
Uppsok
Technology
Supervisors
Examiners
Available from: 2011-06-27 Created: 2011-06-27 Last updated: 2011-06-27Bibliographically approved

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Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab(1483 kB)613 downloads
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Biology Education Centre
Industrial BiotechnologyMedical Laboratory and Measurements TechnologiesBiocatalysis and Enzyme TechnologyImmunology

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CiteExportLink to record
Permanent link

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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
More languages
Output format
  • html
  • text
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