Change search
ReferencesLink to record
Permanent link

Direct link
Comparison of four transport systems for enetric pathogens
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2011 (Swedish)Independent thesis Basic level (degree of Bachelor), 15 credits / 22,5 HE creditsStudent thesis
Abstract [en]

ABSTRACT: Commercial swab transport systems are used for collection and transporting of fecal and other microbiological samples. This system must maintain viability and contribute to survival of microorganisms during transport to the laboratory. Four swab transport systems have been compared, eSwab, Σ- Transwab and fecalSwab, all three with flocked swabs, and Copan Venturi Transystem with rayon swabs. The study followed the recommendations from the Clinical Laboratory Standard Institute; document M40-A for recovery of Samonella, Shigella, Yersinia and Neisseria gonorrhoeae after storage for 0, 6, 24, 48 and 72 h at room- (22-25°C) and refrigerated (2-8ºC) temperature. A fecal sample has also been inoculated with Salmonella or Shigella to simulate a fecal sample positive for Salmonella or Shigella. Recovery of all strains was higher with eSwab, Σ- Transwab and fecalswab than with Copan Venturi Transystem stored at both temperatures. A heavy growth was observed with all transport systems after storage for 24, 48 and 72 h at room temperature, except for Neisseria gonorrhoeae, and Shigella. The number of CFU for all strains was constant up to 72 h at refrigerated temperature with Copan Venturi Transystem. In the experiment with fecal sample recovery of Salmonella and Shigella was best with fecalSwab at both storage temperatures. ESwab,

Σ- Transwab and fecalSwab are equivalent and can be used as an alternative to Copan Venturi Transystem with better survival of enteric pathogens and Neisseria gonorrhoeae. Fecal samples should be refrigerated in order to avoid heavy overgrowth of fecal flora.

Place, publisher, year, edition, pages
2011. , 20 p.
Keyword [en]
Swab transport system; Liquid Amies medium; Fecal sample; Flocked swab
Identifiers
URN: urn:nbn:se:uu:diva-155077OAI: oai:DiVA.org:uu-155077DiVA: diva2:423628
Educational program
Biomedicinska analytikerprogrammet
Uppsok
Medicine
Supervisors
Examiners
Available from: 2011-06-21 Created: 2011-06-15 Last updated: 2011-06-21Bibliographically approved

Open Access in DiVA

fulltext(242 kB)331 downloads
File information
File name FULLTEXT02.pdfFile size 242 kBChecksum SHA-512
3d17724a8089fa5dfc700158893c0c48bd7dd355bcb121a52687275c1f079df9ea576b999de60faa8099070d336bf584664e59c0b1a120c8df13e6f20f3d594e
Type fulltextMimetype application/pdf

By organisation
Department of Medical Biochemistry and Microbiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 331 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 374 hits
ReferencesLink to record
Permanent link

Direct link