Change search
ReferencesLink to record
Permanent link

Direct link
Bioconversion of Heavy oil: Characterizations of Microbial potential to bioconvert Mariner Maureen-, Peregrino- and Bressay oil
Norwegian University of Science and Technology, Faculty of Natural Sciences and Technology, Department of Biotechnology.
2011 (English)MasteroppgaveStudent thesis
Abstract [en]
70 % of world’s oil reservoirs consist of heavy oil, and as the supply of conventional oil decreases, researchers are searching for new technologies to explore and enhance heavy oil recovery. One of the postulated technologies is microbial enhanced oil recovery (MEOR), which is predicted to be a more environmental and economical process for improving oil recovery of heavy oil. The aim of this Master’s project was to give a qualitative indication of three selected consortia’s potential to bioconvert Mariner Maureen-, Peregrino- and Bressay oil. The consortia are comprised of microorganisms isolated from oil sand, mud volcano, processing waters from water treatment plants and oil related samples from other locations. Bioconversion experiments were conducted by inoculating the three selected oils with three consortia. Two terms of different main experimental designs was used; optimal growth temperature of the consortia or the temperature at reservoir conditions, in addition to cultivation with two different growth mediums. After cultivating aerobically for seven days, the oil was separated from the water phase and analyzed by thin-layer chromatography with flame ionization detection (TLC-FID) to identify possible indication of bioconversion. The results demonstrate a reduction in either one or both saturated hydrocarbons and aromatic hydrocarbons of the oil in several of the oil samples. DNA extracted from the water phase that was analyzed with denaturing gradient gel electrophoresis (DGGE), showed several positive results with indication of high biodiversity. The overall results indicate that there has been a microbial impact on some of the heavy oil fractions in Mariner Maureen-, Peregrino- and Bressay oil. Additional experiments must be done to identify the specific changes in the oil and prove microbial impact by repeating these experiments. This could potentially lead to identification of microorganisms with the ability to bioconvert heavy oil and the development of MEOR processes.
Place, publisher, year, edition, pages
Institutt for bioteknologi , 2011. , 139 p.
Keyword [no]
ntnudaim:6466, MBIOT Bioteknologi,
Identifiers
URN: urn:nbn:no:ntnu:diva-12733Local ID: ntnudaim:6466OAI: oai:DiVA.org:ntnu-12733DiVA: diva2:422939
Supervisors
Available from: 2011-06-14 Created: 2011-06-14

Open Access in DiVA

fulltext(4960 kB)1035 downloads
File information
File name FULLTEXT01.pdfFile size 4960 kBChecksum SHA-512
90d1673937185b2bc9eddbb7bd1c26c5a76a97ced11e22a9d8f43fdfc21e4bc4ec2e857d3f995e5bfa1093e681c18d5d741e0f1275e52e28ab77638ac7787671
Type fulltextMimetype application/pdf
cover(101 kB)26 downloads
File information
File name COVER01.pdfFile size 101 kBChecksum SHA-512
dd49cccab9f915b2879c44edb87f0981f951866a7905e67a46cefa8561cc459c330b5fe08e9bc2c96c8581c2430e391600219979e3f7c3e5235e184ba39625e6
Type coverMimetype application/pdf

By organisation
Department of Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 1035 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 94 hits
ReferencesLink to record
Permanent link

Direct link