Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art
2011 (English)In: ASIAN JOURNAL OF ANDROLOGY, ISSN 1008-682X, Vol. 13, no 3, 406-419 p.Article, review/survey (Refereed) Published
Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of bench-top flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.
Place, publisher, year, edition, pages
Blackwell Publishing Ltd , 2011. Vol. 13, no 3, 406-419 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-68353DOI: 10.1038/aja.2011.15ISI: 000290206800015OAI: oai:DiVA.org:liu-68353DiVA: diva2:418133
This is the authors’ version of the following article: Md Sharoare Hossain, Anders Johannisson, Margareta Wallgren, Szabolcs Nagy, Amanda Pimenta Siqueira and Heriberto Rodriguez-Martinez, Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art, 2011, ASIAN JOURNAL OF ANDROLOGY, (13), 3, 406-419. which has been published in final form at: http://dx.doi.org/10.1038/aja.2011.15 Copyright: Blackwell Publishing Ltd http://eu.wiley.com/WileyCDA/Brand/id-35.html2011-05-202011-05-202015-03-12