Change search
ReferencesLink to record
Permanent link

Direct link
Intrinsic Heterogeneity in Liposome Suspensions Caused by the Dynamic Spontaneous Formation of Hydrophobic Active Sites in Lipid Membranes
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
2011 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 27, no 8, 4873-4883 p.Article in journal (Refereed) Published
Abstract [en]

The spontaneous, dynamic formation of hydrophobic active sites in lipid bilayer membranes is studied and characterized. It is shown that the rates of formation and consumption of these active sites control at least two important properties of liposomes: their affinity for hydrophobic surfaces and the rate by which they spontaneously release encapsulated molecules. The adhesion and spreading of liposomes onto hydrophobic polystyrene nanoparticles and the spontaneous leakage of an encapsulated fluorescent dye were monitored for different liposome compositions employing Cryo-TEM, DLS, and fluorescence measurements. It was observed that an apparently homogeneous, monodisperse liposome suspension behaves as if composed by two different populations: a fast leaking population that presents affinity for the hydrophobic substrate employed, and a slow leaking population that does not attach immediately to it. The results reported here suggest that the proportion of liposomes in each population changes over time until a dynamic equilibrium is reached. It is shown that this phenomenom can lead to irreproducibility in, for example, spontaneous leakage experiments, as extruded liposomes leak much faster just after preparation than 24 h afterward. Our findings account for discrepancies in several experimental results reported in the literature. To our knowledge, this is the first systematic study addressing the issue of an existing intrinsic heterogeneity of liposome suspensions.

Place, publisher, year, edition, pages
2011. Vol. 27, no 8, 4873-4883 p.
National Category
Other Basic Medicine
Identifiers
URN: urn:nbn:se:uu:diva-151418DOI: 10.1021/la1049919ISI: 000289321000076PubMedID: 21391645OAI: oai:DiVA.org:uu-151418DiVA: diva2:409822
Available from: 2011-04-18 Created: 2011-04-11 Last updated: 2011-05-03Bibliographically approved

Open Access in DiVA

fulltext(734 kB)354 downloads
File information
File name FULLTEXT01.pdfFile size 734 kBChecksum SHA-512
2d00bc20001857999d788aa57ee82456e28a034343b6cc6265fb1a2e5b33c805989601ef7f734675487773675475a035a60f697199f3fb79df17d185660f231c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Agmo Hernández, VíctorKarlsson, GöranEdwards, Katarina
By organisation
Physical Chemistry
In the same journal
Langmuir
Other Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 354 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 518 hits
ReferencesLink to record
Permanent link

Direct link