Vibrio cholerae cytolysin causes an inflammatory response in human intestinal epithelial cells that is modulated by the PrtV protease
2009 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 4, no 11, e7806- p.Article in journal (Refereed) Published
We suggest that VCC is capable of causing an inflammatory response characterized by increased permeability and production of IL-8 and TNF-alpha in tight monolayers. Pure VCC at a concentration of 160 ng/ml caused an inflammatory response that reached the magnitude of that caused by Vibrio-secreted factors, while higher concentrations caused epithelial cell death. The inflammatory response was totally abolished by treatment with PrtV. The findings suggest that low doses of VCC initiate a local immune defense reaction while high doses lead to intestinal epithelial lesions. Furthermore, VCC is indeed a substrate for PrtV and PrtV seems to execute an environment-dependent modulation of the activity of VCC that may be the cause of V. cholerae reactogenicity.
Place, publisher, year, edition, pages
San Francisco: Public Library of Science , 2009. Vol. 4, no 11, e7806- p.
Immunology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-41945DOI: 10.1371/journal.pone.0007806OAI: oai:DiVA.org:umu-41945DiVA: diva2:408181