Break-induced replication is highly inaccurate
2011 (English)In: PLoS biology, ISSN 1544-9173, E-ISSN 1545-7885Article in journal (Refereed) Published
DNA must be synthesized for purposes of genome duplication and DNA repair. While the former is a highly accurate process, short-patch synthesis associated with repair of DNA damage is often error-prone. Break-induced replication (BIR) is a unique cellular process that mimics normal DNA replication in its processivity, rate, and capacity to duplicate hundreds of kilobases, but is initiated at double-strand breaks (DSBs) rather than at replication origins. Here we employed a series of frameshift reporters to measure mutagenesis associated with BIR in Saccharomyces cerevisiae. We demonstrate that BIR DNA synthesis is intrinsically inaccurate over the entire path of the replication fork, as the rate of frameshift mutagenesis during BIR is up to 2,800-fold higher than during normal replication. Importantly, this high rate of mutagenesis was observed not only close to the DSB where BIR is less stable, but also far from the DSB where the BIR replication fork is fast and stabilized. We established that polymerase proofreading and mismatch repair correct BIR errors. Also, dNTP levels were elevated during BIR, and this contributed to BIR-related mutagenesis. We propose that a high level of DNA polymerase errors that is not fully compensated by error-correction mechanisms is largely responsible for mutagenesis during BIR, with Pol δ generating many of the mutagenic errors. We further postulate that activation of BIR in eukaryotic cells may significantly contribute to accumulation of mutations that fuel cancer and evolution.
Place, publisher, year, edition, pages
dna-polymerase-delta; site-directed mutagenesis; double-strand breaks; ii receptor gene; saccharomyces-cerevisiae; mismatch repair; microsatellite instability; ribonucleotide reductase; translesion synthesis; spontaneous mutations
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-41569DOI: 10.1371/journal.pbio.1000594OAI: oai:DiVA.org:umu-41569DiVA: diva2:406944