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Histone modifications after DNA damage affect survival in Schizosaccharomyces pombe
Södertörn University College, School of Life Sciences. (Karl Ekwall)
2010 (English)Independent thesis Advanced level (degree of Master (One Year)), 60 credits / 90 HE creditsStudent thesisAlternative title
Abstract [en]

S. cerevisiae Ada2 and Bre1 has a role in histone post-translational modifications. Deletion of these genes causes deficiency in acetylation (Ada2) or ubiquitination (Bre1) of histones. Further, mutants lacking these genes or homologous genes showed different phenotypes in human and S. cerevisiae while treated with DNA damaging agents 4-NQO and MMS. Bre1 deficient cells showed 4-NQO sensitivity in S. cerevisiae and resistance in human cells. Since it has been shown that S. pombe is more close to mammals in chromatin regulation we wanted to examine S. pombe response against MMS and 4-NQO. By homologous recombination, genes were deleted and mutants were treated with different concentration of both the genotoxins. In accordance with a previous study, Ada2Δ showed sensitivity to MMS while Brl1Δ & Brl2Δ grew as wild type. Surprisingly, unlike S. cerevisiae, S. pombe showed resistance to 4-NQO and has a phenotype similar to the one found in human cells.

Place, publisher, year, edition, pages
2010. , 22 p.
Keyword [en]
Epigenetics, DNA damage, S. pombe, yeast, histone modification, chromatin, life sciences, gene deletion, gene tagging, pcr, mms, 4-nqo, brl1, brl2
Keyword [un]
Epigenetics، ڈی این اے کو نقصان، ایس pombe، خمیر، histone تبدیلی، chromatin، زندگی سائنس، جین خارج، جین ٹیگنگ، پی سی آر، ایم ایم ایس،
Keyword [sv]
Epigenetik, DNA-skada, S. pombe, jäst, histon modifiering, kromatin biovetenskap, gendeletion, gen taggning, PCR, MMS, 4-nqo, brl1, brl2
National Category
Cell and Molecular Biology
URN: urn:nbn:se:sh:diva-6886OAI: diva2:404653
Available from: 2011-03-18 Created: 2011-03-17 Last updated: 2011-03-18Bibliographically approved

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