Validation of realtime-PCR of Fusarium avenaceum for detection in wheat
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Mould is a common contamination in cereals. The growth of mould can stimulate mycotoxins production andsome of which at critical concentrations cause health problems in humans and animals. Fusarium is one of thefungus species that has been found in crops and can cause major problems for farmers such as reduced harvestand economic losses. A group of Fusarium species, Fusarium avenaceum, Fusarium poae and Fusariumtricinctum express a mycotoxin, enniatin. The limited information available today about enniatin-forming fungiis that they grow out on fields of wheat in colder climates. This project aims at developing methods for detection,quantification and identification of known and unknown fungi present in Swedish cereals during 2009-2011. Theproject was carried out using two previously published methods, TMAV and MGB, which both use TaqManprobes with realtime-PCR detection. The methods were evaluated for robustness, efficiency, accuracy, inclusionand exclusion. The results showed that both methods, TMAV and MGB, could be used to detect Fusariumavenaceum. The results for the TMAV method were that it could be used with custom annealing temperatureand chemical concentrations for the best detection. The MGB method can be used to detect Fusarium avenaceumwith Fusarium tricinctum in the same analysis. Both methods can be included in future mapping projects when itis of interest to quantify enniatin producing moulds.
Place, publisher, year, edition, pages
Mycotoxin, Fungus, Mould, Realtime-PCR, Enniatin
IdentifiersURN: urn:nbn:se:uu:diva-149027OAI: oai:DiVA.org:uu-149027DiVA: diva2:403737